A single centrifugation method for isolating fat droplets from cells and tissues
A single centrifugation method for isolating fat droplets from cells and tissues
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DOI：
10.1194/jlr.d023598
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发表时间：
2012-05-01
影响因子：
6.5
通讯作者：
Wolins, Nathan E.
中科院分区：
文献类型：
--
作者：
Harris, Lydia-Ann L. S.;Shew, Trevor M.;Wolins, Nathan E.
脂肪滴在细胞能量调节中具有重要作用。从细胞或组织中分离FD对于研究这些细胞器仍然很重要。在这里，我们描述了一种程序，其中培养的细胞或组织的整个匀浆在标准微量离心机中用单个离心步骤分级分离。该程序可重复地产生三种高度富集FD、可溶性细胞组分或可沉淀的细胞器/膜的级分。洛杉矶哈里斯L.美国，T. M.休，J.R. Skinner和N. E.狼林从细胞和组织中分离脂肪滴的单次离心方法。J. Lipid Res. 2012. 53：1021-1025。
Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge.jlr This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes.-Harris, L-A. L. S., T. M. Shew, J. R. Skinner, and N. E. Wolins. A single centrifugation method for isolating fat droplets from cells and tissues. J. Lipid Res. 2012. 53: 1021-1025.