The orphan response regulator EpsW is a substrate of the DifE kinase and it regulates exopolysaccharide in Myxococcus xanthus.
The orphan response regulator EpsW is a substrate of the DifE kinase and it regulates exopolysaccharide in Myxococcus xanthus.
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DOI：
10.1038/srep17831
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发表时间：
2015-12-07
影响因子：
4.6
通讯作者：
Yang Z
中科院分区：
文献类型：
--
作者：
Black WP;Wang L;Davis MY;Yang Z
在这里，我们试图确定的DifE组氨酸激酶的下游目标的外泌多糖（EPS）的生产在革兰氏阴性细菌粘球菌的调节。这种细菌是研究IV型菌毛（T4P）的重要模型系统，因为它通过由T4P收缩提供动力的社会（S）运动而运动。EPS对于S运动性是关键的，因为它是该细菌中T4 P收缩的优选锚。先前的研究确定了Dif化学感受途径对EPS产生的调节至关重要。然而，该途径中DifE激酶的下游靶标未知。在这项研究中，EpsW，一个孤儿和单域反应调节剂（RR），被确定为一个潜在的DifE目标首先通过生物信息学。随后的实验表明，epsW是必不可少的EPS生物合成在体内和EpsW直接磷酸化的DifE在体外。epsW的靶向突变表明EpsW不太可能是Dif途径的末端RR。相反，我们认为EpsW是调节M中EPS的多步磷酸化中继的中间体。xanthus
Here we attempted to identify the downstream target of the DifE histidine kinase in the regulation of exopolysaccharide (EPS) production in the Gram-negative bacterium Myxococcus xanthus. This bacterium is an important model system for the studies of Type IV pilus (T4P) because it is motile by social (S) motility which is powered by T4P retraction. EPS is critical for S motility because it is the preferred anchor for T4P retraction in this bacterium. Previous studies identified the Dif chemosensory pathway as crucial for the regulation of EPS production. However, the downstream target of the DifE kinase in this pathway was unknown. In this study, EpsW, an orphan and single-domain response regulator (RR), was identified as a potential DifE target first by bioinformatics. Subsequent experiments demonstrated that epsW is essential for EPS biosynthesis in vivo and that EpsW is directly phosphorylated by DifE in vitro. Targted mutagenesis of epsW suggests that EpsW is unlikely the terminal RR of the Dif pathway. We propose instead that EpsW is an intermediary in a multistep phosphorelay that regulates EPS in M. xanthus.