Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women
Regulators of blood lipids and lipoproteins? PPARδ and AMPK, induced by exercise, are correlated with lipids and lipoproteins in overweight/obese men and women
复制标题
DOI：
10.1152/ajpendo.00309.2012
复制
发表时间：
2012-11-01
影响因子：
5.1
通讯作者：
Crouse, Stephen F.
中科院分区：
文献类型：
--
作者：
Greene, Nicholas P.;Fluckey, James D.;Crouse, Stephen F.
过氧化物酶体增殖物激活受体δ（PPARdelta）是一种调节氧化代谢相关基因表达的转录因子，可通过氧化和脂蛋白代谢基因的转录调节血胆固醇水平。为了确定运动前和运动后骨骼肌中过氧化物酶体增殖物激活受体δ含量与血脂和脂蛋白的关系，招募了超重和肥胖的男性（n = 9）和女性（n = 7）;年龄、BMI、体脂百分比和V（O2 max）（平均值+/- SE）分别为45 +/- 2.5年、31.9 +/- 1.4 kg/m（-2）、41.1 +/-1.5%和26.0 +/- 1.3 mLO（2）.kg（-1）.min（-1）。受试者进行了12周的耐力运动训练（3次/周，进展到500千卡/次）。为了评估急性运动反应，受试者在训练前后在跑步机上进行单次运动（70% V-O2 max，400 kcal能量消耗）。肌肉和血液样本之前，任何运动和24小时后，每个急性运动会议。分析肌肉中的蛋白质含量，包括过氧化物酶体增殖物激活受体δ、过氧化物酶体增殖物激活受体α、PGC-1 α、AMPK α以及氧化和脂蛋白标记物FAT/CD 36、CPT I、COX-4、LPL、F-1 ATP酶、ABCAI和LDL受体。评估血液中的脂质和脂蛋白。重复测量方差分析显示，性别对测量结果没有影响。急性运动后，PPAR δ、PGC-1 α、FAT/CD 36和LPL含量增加，而PPAR α、AMPK α、CPT I和COX-IV含量仅在运动训练后增加。在非训练状态下，PPARdelta含量与总胆固醇和LDL胆固醇呈显著负相关（r = 0.5543，P < 0.05）。我们的研究结果表明，运动诱导的骨骼肌PPARs及其靶蛋白的表达，这种表达与肥胖成年人血脂和脂蛋白的改善有关。
PPAR delta is a transcription factor regulating the expression of genes involved in oxidative metabolism, which may regulate blood cholesterols through transcription of oxidative and lipoprotein metabolism genes. To determine the association of skeletal muscle PPAR delta content with blood lipids and lipoproteins before and following exercise, overweight and obese men (n = 9) and women (n = 7) were recruited; age, BMI, body fat percentage, andV(O2max) were (means +/- SE) 45 +/- 2.5 yr, 31.9 +/- 1.4 kg/m(-2), 41.1 +/- 1.5%, and 26.0 +/- 1.3 mLO(2).kg(-1).min(-1), respectively. Subjects performed 12 wk of endurance exercise training (3 sessions/wk, progressing to 500 kcal/session). To assess the acute exercise response, subjects performed a single exercise session on a treadmill (70% V-O2max, 400 kcal energy expenditure) before and after training. Muscle and blood samples were obtained prior to any exercise and 24 h after each acute exercise session. Muscle was analyzed for protein content of PPAR delta, PPAR alpha, PGC-1 alpha, AMPK alpha, and the oxidative and lipoprotein markers FAT/CD36, CPT I, COX-IV, LPL, F-1 ATPase, ABCAI, and LDL receptor. Blood was assessed for lipids and lipoproteins. Repeated-measures ANOVA revealed no influence of sex on measured outcomes. PPAR delta, PGC-1 alpha, FAT/CD36, and LPL content were enhanced following acute exercise, whereas PPAR alpha, AMPK alpha, CPT I, and COX-IV content were enhanced only after exercise training. PPAR delta content negatively correlated with total and LDL cholesterol concentrations primarily in the untrained condition (r = 0.5543, P < 0.05). Our findings demonstrate exercise-induced expression of skeletal muscle PPARs and their target proteins, and this expression is associated with improved blood lipids and lipoproteins in obese adults.