Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular Phone.
Quantitative Fluorescence Assays Using a Self-Powered Paper-Based Microfluidic Device and a Camera-Equipped Cellular Phone.
复制标题
DOI：
10.1039/c3ra44717k
复制
发表时间：
2014-01-01
期刊：
影响因子：
3.9
通讯作者：
Phillips ST
中科院分区：
文献类型：
--
作者：
Thom NK;Lewis GG;Yeung K;Phillips ST
荧光分析通常需要专门的设备，因此在资源有限的环境中不容易实施。在这里，我们描述了一种护理点分析策略，其中可见光区域的荧光被用作读数，而配备相机的手机被用来捕捉荧光响应并对分析进行量化。荧光分析是使用纸基微流控设备实现的，该设备包含一个内部流体电池、一个表面贴装LED、一段2毫米长的透明吸管作为试管，以及一个设计适当的小分子试剂，当接触到特定的酶生物标记物时，小分子试剂可以从弱荧光转变为强荧光。由此产生的可见荧光是通过使用配备相机的手机拍摄化验区域而数字化的。然后使用图像处理软件对数字图像进行量化，以提供敏感的和定量的结果。在模型30分钟的试验中，β-D-半乳糖苷酶被定量测定到700 PM水平。本通信描述了纸基微流控装置中这些类型的分析的设计，并描述了影响该技术的灵敏度和重复性的关键参数。
Fluorescence assays often require specialized equipment and, therefore, are not easily implemented in resource-limited environments. Herein we describe a point-of-care assay strategy in which fluorescence in the visible region is used as a readout, while a camera-equipped cellular phone is used to capture the fluorescent response and quantify the assay. The fluorescence assay is made possible using a paper-based microfluidic device that contains an internal fluidic battery, a surface-mount LED, a 2-mm section of a clear straw as a cuvette, and an appropriately-designed small molecule reagent that transforms from weakly fluorescent to highly fluorescent when exposed to a specific enzyme biomarker. The resulting visible fluorescence is digitized by photographing the assay region using a camera-equipped cellular phone. The digital images are then quantified using image processing software to provide sensitive as well as quantitative results. In a model 30 min assay, the enzyme β-D-galactosidase was measured quantitatively down to 700 pM levels. This Communication describes the design of these types of assays in paper-based microfluidic devices and characterizes the key parameters that affect the sensitivity and reproducibility of the technique.