Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
Protein-binding aptamer assisted signal amplification for the detection of influenza A (H1N1) DNA sequences based on quantum dot fluorescence polarization analysis.
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DOI：
10.1039/c3an00830d
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发表时间：
2013-07
期刊：
影响因子：
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通讯作者：
Juanni Zhang;Jianniao Tian;Yan-Hui He;Sheng Chen;Yixuan Jiang;Yanchun Zhao;Shulin Zhao
中科院分区：
文献类型：
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作者：
Juanni Zhang;Jianniao Tian;Yan-Hui He;Sheng Chen;Yixuan Jiang;Yanchun Zhao;Shulin Zhao
我们报道了一种基于DNA功能量子点荧光偏振探针和双功能蛋白结合适体（Apt-DNA）的H1N1检测荧光偏振平台。该检测方法的线性范围为10 nM至100 nM，检测限为3.45 nM，对错配碱基具有选择性。
We report a fluorescence polarization platform for H1N1 detection based on the construction of a DNA functional QD fluorescence polarization probe and a bi-functional protein binding aptamer (Apt-DNA). The assay has a linear range from 10 nM to 100 nM with a detection limit of 3.45 nM and is selective over the mismatched bases.