Phosphoenolpyruvate carboxykinase revisited - Insights into its metabolic role
Phosphoenolpyruvate carboxykinase revisited - Insights into its metabolic role
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DOI：
10.1002/bmb.2002.494030010018
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发表时间：
2002-01-01
影响因子：
1.4
通讯作者：
Hanson, RW
中科院分区：
文献类型：
--
作者：
Croniger, CM;Olswang, Y;Hanson, RW
我们对主要代谢途径的理解建立在追溯到生物化学开始的坚实的研究记录上。例如，在哺乳动物身上描述利用主要代谢燃料的新途径是不太可能的。然而，不太确定的是，构成代谢途径的酶反应复杂网络的调节机制是什么。例如，脂肪酸和碳水化合物代谢之间相互作用的细节仍有待充分阐明。然而，新的见解改变了我们看待代谢途径关键步骤的方式。正如我们将在这篇文章中阐述的那样，曾经被认为对一种代谢途径很重要的酶，现在被证明对另一种代谢途径是关键的。这主要是因为分子遗传学和体内示踪方法在过去30年中取得的进展，为新陈代谢研究提供了一套新的工具。这一结果极大地扩展了我们对单个酶在特定代谢途径中所起作用的理解。在这篇综述中，我们将介绍磷酸烯醇式丙酮酸羧激酶(PEPCK)1在中间代谢中的生物学作用的最新信息。自20世纪50年代末发现以来，PEPCK一直被认为是肝和肾糖异生的关键步骤。它被广泛认为是糖尿病的关键酶，因为它的活性的升高与
Our understanding of the major metabolic pathways is built on a solid record of research extending back to the beginnings of biochemistry. It is unlikely, for example, that a new pathway for the utilization of major metabolic fuels will be described in mammals. What is less certain, however, are the mechanisms responsible for the regulation of the complex networks of enzymatic reactions that make up a metabolic pathway. As an example, the details of the interaction between fatty acid and carbohydrate metabolism remain to be fully clarified. However, new insights have changed the way we view key steps in metabolic pathways. As we shall develop in this article, enzymes once considered important for one metabolic pathway are now being shown to be critical for another. This is primarily because of advances made in molecular genetics and in vivo tracer methods over the past three decades that have provided a new set of tools for the study of metabolism. The result has greatly extended our understanding of the role played by individual enzymes in specific metabolic pathways.In this review we will present the latest information on the biological role of the enzyme phosphoenolpyruvate carboxykinase (PEPCK) 1 in intermediary metabolism. Since its discovery in the late 1950s, PEPCK has been recognized as a key step in hepatic and renal gluconeogenesis. It is widely recognized as a critical enzyme in diabetes, because an elevation in its activity is associated