ULTRASTRUCTURAL LOCALIZATION OF ALKALINE PHOSPHATASE ACTIVITY IN THE ABSORBING CELLS OF THE DUODENUM OF MOUSE
ULTRASTRUCTURAL LOCALIZATION OF ALKALINE PHOSPHATASE ACTIVITY IN THE ABSORBING CELLS OF THE DUODENUM OF MOUSE
复制标题
小鼠十二指肠吸收细胞碱性磷酸酶活性的超微结构定位
DOI：
10.1177/14.9.629
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发表时间：
1966
影响因子：
3.2
通讯作者：
M. Borgers
中科院分区：
文献类型：
--
作者：
J. Hugon;M. Borgers
用直接铅法显示碱性磷酸酶在pH为9时的活性，对小鼠十二指肠吸收细胞进行了研究。在戊二醛固定的冰冻切片中孵育一小段时间后，在几种结构中观察到了磷酸铅沉淀。在微绒毛和终末纤网下的液泡中，反应强烈，即使在渗酸固定后仍持续存在。在高尔基带、光滑网状池和致密小体中，反应明显。在这些部位，渗酸固定后或在含有β-甘油磷酸或α-萘磷酸以外的底物的介质中短期孵育后，未观察到沉积。
A direct lead method for the visualization of alkaline phosphatase activity at pH 9 was used in a study on the absorptive cells of the duodenum of the mouse. Lead phosphate precipitate was observed in several structures after a short incubation of glutaraldehyde-fixed frozen sections. On microvilli and in vacuoles below the terminal web, the reaction was intense and persisted even after osmic acid fixation. In the Golgi zone, in the smooth reticulum cisternae and in the dense bodies, the reaction was prominent. In these sites, the deposits were not observed after osmic acid fixation or after short incubation in media containing substrates other than β-glycerophosphate or α-naphthylphosphate.