OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping.
OMIP-098: A 26 parameter, 24 color flow cytometry panel for human memory NK cell phenotyping.
复制标题
OMIP-098：用于人类记忆 NK 细胞表型分析的 26 参数、24 色流式细胞仪面板。
DOI：
10.1002/cyto.a.24802
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Machmach,Kawthar
中科院分区：
文献类型：
--
作者：
Creegan,Matthew;Degler,Justin;Paquin-Proulx,Dominic;Eller,MichaelA;Machmach,Kawthar
2 背景自然杀伤(NK) 细胞是先天免疫系统的组成部分。它们与 T 细胞，特别是 CD8 T 细胞 [1] 相当，既可作为细胞毒性效应细胞，又在抗病毒和抗肿瘤免疫反应中发挥着至关重要的作用。然而，它们在识别、特异性和记忆机制方面有所不同。 CD8 T 细胞利用其 T 细胞抗原受体识别抗原呈递细胞表面的肽主要组织相容性复合物，随后触发其激活、分化和功能。另一方面，NK 细胞可以在没有事先致敏的情况下执行快速的细胞溶解和免疫调节功能。一旦被激活，NK 细胞可以通过多种机制帮助清除病毒感染的细胞或肿瘤细胞，包括通过释放穿孔素、颗粒酶、免疫调节细胞因子和趋化因子来直接细胞毒性，或通过与 T 细胞和树突状细胞的串扰来间接影响适应性免疫反应 [2-6]。
2 BACKGROUNDNatural killer (NK) cells are components of the innate immune system. They are comparable to T cells, in particular CD8 T cells [1], serving both as cytotoxic effector cells and playing a crucial role in anti-viral and anti-tumor immune responses. However, they differ in recognition, specificity, and memory mechanisms. CD8 T cells use their T cell antigen receptor to recognize the peptide-major histocompatibility complex on the surface of the antigen presenting cells, and subsequently trigger their activation, differentiation, and function. NK cells on the other hand can perform rapid cytolytic and immunomodulatory functions in the absence of prior sensitization. Once activated, NK cells can help clear virus-infected or tumor cells through multiple mechanisms including direct cytotoxicity by releasing perforin, granzyme, immunoregulatory cytokines, and chemokines, or indirectly by influencing adaptive immune responses through their crosstalk with T and dendritic cells [2-6].