The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs
The phosphorylation of Tudor-SN mediated by JNK is involved in the regulation of milk protein synthesis induced by prolactin in BMECs
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JNK介导的Tudor-SN磷酸化参与BMEC中催乳素诱导的乳蛋白合成的调节
DOI：
10.1002/jcp.27343
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发表时间：
2019
影响因子：
5.6
通讯作者：
Ao Jin Xia
中科院分区：
文献类型：
--
作者：
Zhang Sen;Qi Hao;Wen Xue Peng;Li Ping;Gao Xue Jun;Ao Jin Xia
Tudor葡萄球菌核酸酶（Tudor‐SN）是一种参与多种细胞过程的多功能蛋白，在基因表达调控中起关键作用。最近，Tudor‐SN在哺乳期间被发现在乳腺上皮细胞中响应催乳素而上调，从而进一步调节乳蛋白的合成。然而，Tudor‐SN对乳蛋白的具体调控机制仍有待阐明。在我们的研究中，我们观察到在催乳素刺激下Tudor - SN和phosphor - Tudor - SN （Thr103）的水平均升高。免疫荧光分析表明，催乳素治疗促进了Tudor‐SN的核运输。进一步的研究表明Tudor‐SN的磷酸化依赖于活化的JNK。共免疫沉淀实验显示Tudor‐SN可能被JNK直接磷酸化。通过基因突变分析，我们进一步发现Thr到Ala在103位点的突变阻止了Tudor‐SN的核转运。因此，这些结果揭示了Tudor‐SN在乳蛋白响应催乳素调控中的重要作用机制，为提高乳蛋白产量提供了新的思路。
Tudor staphylococcal nuclease (Tudor‐SN) is a multifunctional protein involved in a variety of cellular processes and plays a critical role in the regulation of gene expression. Recently, Tudor‐SN was found to be upregulated in mammary epithelial cells during lactation in response to prolactin, which further to regulate milk protein synthesis. However, the detailed regulatory mechanism of Tudor‐SN to milk protein still remains to be elucidated. In our study, we observed that the levels of Tudor‐SN and phosphor‐Tudor‐SN (Thr103) were both enhanced upon prolactin stimulation. Immunofluorescence assays demonstrated that prolactin treatment facilitated the nuclear transport of Tudor‐SN. Further study revealed that the phosphorylation of Tudor‐SN was depended on activated JNK. Coimmunoprecipitation assays disclosed that Tudor‐SN might be phosphorylated directly by JNK. Using gene mutation assays, we further discovered that mutation of Thr to Ala at site of 103 prevented the nuclear transport of Tudor‐SN. Thus, these results suggested the essential mechanism of the activated Tudor‐SN in milk protein regulation in response to prolactin, which may provide some new sights into improve milk protein production.