Camellia oil (Camellia oleifera Abel.) attenuates CCl4-induced liver fibrosis via suppressing hepatocyte apoptosis in mice
Camellia oil (Camellia oleifera Abel.) attenuates CCl4-induced liver fibrosis via suppressing hepatocyte apoptosis in mice
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DOI：
10.1039/c9fo02258a
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发表时间：
2020-05-01
期刊：
影响因子：
6.1
通讯作者：
Xu, Xundi
中科院分区：
文献类型：
--
作者：
Lei, Xiaohua;Liu, Qiang;Xu, Xundi
肝纤维化是许多慢性肝病病理发展的共同部分。随着肝纤维化的进展，它可能导致肝硬化、门静脉高压、肝失代偿、肝肿瘤和死亡。山茶油（Camellia oleifera Abel.）在中国被广泛用作食用油。它具有广泛的生物活性，被用作治疗烧伤和胃痛等疾病的传统药物。然而，山茶油是否可以改善肝纤维化仍不清楚。建立四氯化碳（CCl 4）诱导的大鼠肝纤维化模型，通过生化、组织病理形态学、免疫组化和Western blot分析，证实山茶油在肝纤维化中的作用。我们发现，山茶油改善组织病理学病变，改善肝功能和抗氧化能力，降低TGF-β 1和α-SMA蛋白的表达，下调CCl 4诱导的肝纤维化中促凋亡蛋白的表达。因此，这些结果表明，山茶油减轻四氯化碳诱导的小鼠肝纤维化，其机制可能是通过减少肝细胞凋亡，抑制肝星状细胞（HSC）的激活。山茶油可能提供一种潜在的新的治疗肝纤维化的辅助治疗，通过添加食用油到日常饮食。
Liver fibrosis is a common part of the pathological development of many chronic liver diseases. As liver fibrosis progresses, it may lead to cirrhosis, portal hypertension, liver decompensation, liver tumours, and death. Camellia oil (Camellia oleifera Abel.) is widely used as an edible oil in China. It has a wide range of biological activities and is used as a traditional medicine to treat conditions such as burns and stomach pains. However, whether camellia oil can ameliorate liver fibrosis remains unclear. We constructed a liver fibrosis model induced by carbon tetrachloride (CCl4) and then confirmed the role of camellia oil in liver fibrosis by biochemical examination, histopathological morphology, immunohistochemistry, and western blot analysis. We found that camellia oil ameliorated histopathological lesions, improved liver function and antioxidant capacity, decreased the expression of TGF-beta 1 and alpha-SMA proteins, and downregulated the expression of the pro-apoptotic proteins in CCl4-induced liver fibrosis. Therefore, these results suggest that camellia oil attenuates CCl4-induced liver fibrosis in mice, and its mechanism may function via reduction of hepatocyte apoptosis to inhibit hepatic stellate cell (HSC) activation. Camellia oil may provide a potential new treatment for liver fibrosis as an auxiliary treatment by addition of the edible oil to the daily diet.