A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
A Sialic Acid-Binding Protein SABP1 of Toxoplasma gondii Mediates Host Cell Attachment and Invasion
复制标题
弓形虫唾液酸结合蛋白 SABP1 介导宿主细胞附着和侵袭
DOI：
10.1093/infdis/jiaa072
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发表时间：
2020-07-01
影响因子：
6.4
通讯作者：
Chen，Qijun
中科院分区：
文献类型：
--
作者：
Xing，Mengen;Yang，Na;Chen，Qijun
摘要许多专性胞内顶复门寄生虫具有独特的入侵机制，包括寄生虫配体与唾液酸受体之间的密切相互作用。我们发现，唾液酸结合蛋白1（SABP 1），定位于外膜的人畜共患寄生虫弓形虫，很容易结合到宿主细胞表面的唾液酸。这种结合对神经氨酸酶处理敏感。用重组SABP 1蛋白预孵育的细胞在体外抵抗寄生虫侵袭。在SABP1基因缺失后，该寄生虫失去了其入侵能力和动物感染性，而SABP1基因的互补恢复了敲除菌株的毒力。这些数据证实了SABP 1在T.刚地。SABP 1是一种以前未被鉴定的蛋白质，它促进了T.弓形虫通过唾液酸受体附着和侵入。
Abstract Many obligate intracellular apicomplexan parasites have adapted a distinct invasion mechanism involving a close interaction between the parasite ligands and the sialic acid (SA) receptor. We found that sialic acid binding protein-1 (SABP1), localized on the outer membrane of the zoonotic parasite Toxoplasma gondii, readily binds to sialic acid on the host cell surface. The binding was sensitive to neuraminidase treatment. Cells preincubated with recombinant SABP1 protein resisted parasite invasion in vitro. The parasite lost its invasion capacity and animal infectivity after the SABP1 gene was deleted, whereas complementation of the SABP1 gene restored the virulence of the knockout strain. These data establish the critical role of SABP1 in the invasion process of T. gondii. The previously uncharacterized protein, SABP1, facilitated T. gondii attachment and invasion via sialic acid receptors.