Psychological stress induced zinc accumulation and up-regulation of ZIP14 and metallothionein in rat liver.
Psychological stress induced zinc accumulation and up-regulation of ZIP14 and metallothionein in rat liver.
复制标题
心理应激诱导大鼠肝脏锌积累及ZIP14和金属硫蛋白上调
DOI：
10.1186/1471-230x-14-32
复制
发表时间：
2014-02-18
影响因子：
2.4
通讯作者：
Shen H
中科院分区：
文献类型：
--
作者：
Tian X;Zheng Y;Li Y;Shen Z;Tao L;Dou X;Qian J;Shen H
锌是正常肝功能所必需的；反之亦然，肝脏在锌的体内平衡中起着核心作用。本研究旨在探讨反复心理应激对大鼠肝脏锌代谢的影响及锌稳态的相关机制。方法本研究采用通讯箱建立PS模型，检测大鼠血清皮质酮（CORT）、血清及肝脏锌水平、肝脏金属硫蛋白（MT）含量及ZRT/ irt样蛋白14 (ZIP14) mRNA表达。结果经7 d和14 d PS处理后，大鼠血清CORT水平显著升高，血清锌水平显著降低。同时，暴露14 d后肝脏锌和MT含量升高，而暴露7 d后肝脏锌和MT含量无变化。ZIP14 mRNA在PS发病后第7天显著表达，而在PS暴露后第14天肝脏中ZIP14 mRNA的表达量与对照组相比达到正常水平。结论PS暴露可引起低锌血症，可能与糖皮质激素介导MT合成和白细胞介素-6诱导的ZIP14表达导致MT水平升高导致肝脏锌积累有关。
BackgroundZinc is necessary for normal liver function; and vice versa, the liver plays a central role in zinc homeostasis. The aim of present study is to assess the effects of repeated psychological stress (PS) on the zinc metabolism and related mechanism involved in zinc homeostasis in rat liver.MethodsIn present study, we used communication box to create PS model and investigated the serum corticosterone (CORT), zinc level in serum and liver, liver metallothionein (MT) content and ZRT/IRT-like Protein 14 (ZIP14) mRNA expression.ResultsThe results showed that the serum CORT level increased and serum zinc level decreased significantly after 7 d and 14 d PS treatment. Meanwhile, zinc and MT contents in liver were elevated after 14 d PS exposure, while those in 7 d PS exposure group did not change. ZIP14 mRNA was expressed markedly at 7 d after the onset of PS, while Zip14 mRNA expression in the liver after 14 d PS exposure reached normal level compared with control group.ConclusionsThe results suggest that PS exposure could induce hypozincemia, which might be related to liver zinc accumulation because of high level of MT through glucocorticoid-mediated MT synthesis and ZIP14 expression induced by interleukin-6.