Inhibitory function of P-selectin-mediated leukocyte adhesion by the polysaccharides from Sanguisorba officinalis
Inhibitory function of P-selectin-mediated leukocyte adhesion by the polysaccharides from Sanguisorba officinalis
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地榆多糖对P-选择素介导的白细胞粘附的抑制作用
DOI：
10.3109/13880209.2014.919597
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发表时间：
2015-01
影响因子：
3.8
通讯作者：
Jiang Guiquan
中科院分区：
文献类型：
--
作者：
Tong Haibin;Song Jianxi;Zhang Zhuorui;Mao Dirui;Sun Guangren;Jiang Guiquan
Abstract Context: P-selectin is a promising target for inflammatory-related diseases. Polysaccharides are the active ingredients of Sanguisorba officinalis L. (Rosaceae) responsible for its anti-inflammatory activities; however, the molecular mechanism is not clear yet. Objective: This study evaluates the effects of polysaccharides (SOPs) from Sanguisorba officinalis on their antagonistic function against P-selectin-mediated leukocyte adhesion. Materials and methods: The antagonistic function of SOPs was investigated by flow cytometry and static adhesion assay at the concentrations of 25 and 100 μg/ml. The dynamic interaction between HL-60 cells and CHO-P cell monolayer treated with SOPs (25 and 100 μg/ml) was analyzed in a parallel plate flow chamber, and quantitatively calculated by ImageJ software (NIH, Bethesda, MD). In vitro protein binding assay was carried out to evaluate the blocking effects of SOPs (25 and 100 μg/ml) on the interaction between P-selectin and PSGL-1. Results: SOPs-treatment (100 μg/ml) significantly reduced the percentage of HL-60 cells binding to P-selectin (p < 0.01) determined by flow cytometry. In addition, SOPs (25 and 100 μg/ml) markedly blocked the adhesion between HL-60 cells and CHO-P cells under static condition, and the inhibitory rates reached 39.9% and 71.2%, respectively. Compared with the positive control group, SOPs-treatment (25 and 100 μg/ml) significantly reduced the percentage of HL-60 cells rolling on CHO-P cell monolayers by 43.5% and 75.2%, respectively. Protein binding assay showed the interaction between P-selectin and PSGL-1 was significantly blocked by SOPs. Discussion and conclusion: SOPs possess a significant antagonistic function against P-selectin-mediated leukocyte adhesion, and SOPs could be considered as a promising candidate for amelioration of inflammation-related diseases.
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影响因子：
2
作者：
Nguyen, Thi Thuy Ha;Cho, Soon Ock;Seong, Yeon Hee
通讯作者：
Seong, Yeon Hee
影响因子：
4.6
作者：
M. E. Preobrazhenskaya;A. Berman;V. I. Mikhailov;N. A. Ushakova;A. Mazurov;A. Semenov;A. I. Usov;N. Nifant’ev;N. Bovin
通讯作者：
M. E. Preobrazhenskaya;A. Berman;V. I. Mikhailov;N. A. Ushakova;A. Mazurov;A. Semenov;A. I. Usov;N. Nifant’ev;N. Bovin
柴胡多糖通过阻断 fMLP 趋化受体介导的功能来影响中性粒细胞的招募和迁移
影响因子：
11.2
作者：
Tong, Haibin;Tian, Dan;Sun, Xin
通讯作者：
Sun, Xin
影响因子：
5.4
作者：
Lee, Nam-Hun;Lee, Mee-Young;Shin, Hyeun-Kyoo
通讯作者：
Shin, Hyeun-Kyoo
影响因子：
6.4
作者：
P. Kubes;P. Ward
通讯作者：
P. Kubes;P. Ward