Transient formation of DNA strand breaks during the induced differentiation of a human promyelocytic leukaemic cell line, HL-60.
Transient formation of DNA strand breaks during the induced differentiation of a human promyelocytic leukaemic cell line, HL-60.
复制标题
在人类早幼粒细胞白血病细胞系 HL-60 的诱导分化过程中，DNA 链断裂的瞬时形成。
作者：
F. Farzaneh;R. Meldrum;S. Shall
在人早幼粒细胞白血病细胞系HL-60的诱导分化过程中，沿着粒细胞谱系形成DNA链断裂。这些断裂是在熟练的DNA修复机制面前形成的，只是暂时维持，随后重新连接。这些断裂的连接需要核腺苷二磷酸核糖转移酶（ADPRT）的活性。核ADPRT是一种酶，其活性完全依赖于DNA链断裂的存在，并且是真核细胞中有效DNA修复所需的，其抑制阻断了这些断裂的重新连接，但不阻断其形成。在分化的HL-60细胞中抑制DNA链连接导致活力丧失和细胞死亡。
During the induced differentiation of the human promyelocytic leukaemic cell line, HL-60, along the myelocytic lineage, DNA strand-breaks are formed. These breaks which are formed in the face of a proficient DNA repair mechanism, are only transiently maintained and subsequently become religated. The ligation of these breaks requires the activity of the nuclear adenosine diphosphoribosyl transferase (ADPRT). Inhibition of nuclear ADPRT, an enzyme totally dependent on the presence of DNA strand-breaks for its activity and required for efficient DNA repair in eukaryotic cells, blocks the religation of these breaks but not their formation. The inhibition of DNA strand ligation in the differentiating HL-60 cells results in loss of viability and cell death.