Comparative transcriptome profiling of horseshoe crab Tachypleus gigas hemocytes in response to lipopolysaccharides
Comparative transcriptome profiling of horseshoe crab Tachypleus gigas hemocytes in response to lipopolysaccharides
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DOI：
10.1016/j.fsi.2021.08.001
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发表时间：
2021-08-07
影响因子：
4.7
通讯作者：
Yaacob，Nik Soriani
中科院分区：
文献类型：
--
作者：
Sarmiento，Maria E.;Chin，Kai Ling;Yaacob，Nik Soriani
马蹄蟹是海洋节肢动物的活化石，具有约5亿年的进化历史。它们的生存受到先天免疫系统的帮助，该系统包括细胞和体液免疫成分，以保护它们免受入侵病原体的侵害。为了帮助理解所涉及的遗传机制，本研究利用Illumina HiSeq平台对用脂多糖（LPS）挑战的HSC（Tachypleus gigas）的血细胞进行转录组学分析。高通量测序得到352，077，208和386，749，136个原始读段，分别对应于对照和LPS处理的血细胞样品的282，490，910和305，709，830个高质量可映射读段。根据对数倍数变化>0.3或<-0.3，LPS刺激后1338个基因显著上调，215个基因显著下调。这些差异表达基因（DEG）与免疫防御、应激反应、细胞骨架功能和信号转导等多种途径相关。这项研究提供了深入了解暴露于LPS的血细胞中的潜在分子和调控机制，这与HSC对感染的免疫反应的研究有关。
Horseshoe crabs (HSCs) are living fossil species of marine arthropods with a long evolutionary history spanning approximately 500 million years. Their survival is helped by their innate immune system that comprises cellular and humoral immune components to protect them against invading pathogens. To help understand the genetic mechanisms involved, the present study utilised the Illumina HiSeq platform to perform transcriptomic analysis of hemocytes from the HSC, Tachypleus gigas, that were challenged with lipopolysaccharides (LPS). The high-throughput sequencing resulted in 352,077,208 and 386,749,136 raw reads corresponding to 282,490,910 and 305,709,830 high-quality mappable reads for the control and LPS-treated hemocyte samples, respectively. Based on the log-fold change of>0.3 or < -0.3, 1338 genes were significantly upregulated and 215 genes were significantly downregulated following LPS stimulation. The differentially expressed genes (DEGs) were further identified to be associated with multiple pathways such as those related to immune defence, stress response, cytoskeleton function and signal transduction. This study provides insights into the underlying molecular and regulatory mechanisms in hemocytes exposed to LPS,which has relevance for the study of the immune response of HSCs to infection.