Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156.
Sucrose and ABA regulate starch biosynthesis in maize through a novel transcription factor, ZmEREB156.
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蔗糖和 ABA 通过新型转录因子 ZmEREB156 调节玉米淀粉生物合成
DOI：
10.1038/srep27590
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发表时间：
2016-06-10
影响因子：
4.6
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Huang H;Xie S;Xiao Q;Wei B;Zheng L;Wang Y;Cao Y;Zhang X;Long T;Li Y;Hu Y;Yu G;Liu H;Liu Y;Huang Z;Zhang J;Huang Y
蔗糖不仅是淀粉合成的碳源，也是信号分子。单独或与ABA协同调节淀粉合成相关基因的表达。为了研究这种效应背后的分子机制，在授粉后 10 天从 Zea mays L. B73 自交系收集玉米胚乳，并用蔗糖、ABA 或蔗糖加 ABA 在 28°C 黑暗中处理 24 小时。玉米胚乳转录组的 RNA 序列分析揭示了差异表达基因中的 47 个候选转录因子。因此，我们推测淀粉合成基因的表达受到蔗糖和ABA结合诱导的转录因子的调节。 ZmEREB156 是一种候选转录因子，由蔗糖和 ABA 诱导，参与淀粉生物合成。 ZmEREB156-GFP融合蛋白定位于洋葱表皮细胞的细胞核中，并且ZmEREB156蛋白具有很强的转录激活活性。在玉米胚乳中过表达ZmEREB156后，淀粉相关基因Zmsh2和ZmSSIIIa的启动子活性增加。在酵母单杂交系统中，ZmEREB156 可以结合 ZmSSIIIa 启动子，但不能结合 Zmsh2 启动子。因此，ZmEREB156通过蔗糖和ABA的协同作用正向调节淀粉生物合成基因ZmSSIIIa。
Sucrose is not only the carbon source for starch synthesis, but also a signal molecule. Alone or in coordination with ABA, it can regulate the expression of genes involved in starch synthesis. To investigate the molecular mechanisms underlying this effect, maize endosperms were collected from Zea mays L. B73 inbred line 10 d after pollination and treated with sucrose, ABA, or sucrose plus ABA at 28 °C in the dark for 24 h. RNA-sequence analysis of the maize endosperm transcriptome revealed 47 candidate transcription factors among the differentially expressed genes. We therefore speculate that starch synthetic gene expression is regulated by transcription factors induced by the combination of sucrose and ABA. ZmEREB156, a candidate transcription factor, is induced by sucrose plus ABA and is involved in starch biosynthesis. The ZmEREB156-GFP-fused protein was localized in the nuclei of onion epidermal cells and ZmEREB156 protein possessed strong transcriptional activation activity. Promoter activity of the starch-related genes Zmsh2 and ZmSSIIIa increased after overexpression of ZmEREB156 in maize endosperm. ZmEREB156 could bind to the ZmSSIIIa promoter but not the Zmsh2 promoter in a yeast one-hybrid system. Thus, ZmEREB156 positively modulates starch biosynthetic gene ZmSSIIIa via the synergistic effect of sucrose and ABA.