The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on endoplasmic reticulum stress and apoptosis in chicken spleen induced by heat stress
The effect of selenium and polysaccharide of Atractylodes macrocephala Koidz. (PAMK) on endoplasmic reticulum stress and apoptosis in chicken spleen induced by heat stress
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硒和白术多糖的作用。
DOI：
10.1039/c6ra27730f
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发表时间：
2017-01
期刊：
影响因子：
--
通讯作者：
Yunbo Tian
中科院分区：
文献类型：
--
作者：
Danning Xu;Wanyan Li;Bingxin Li;Yunbo Tian
内质网应激和氧化应激参与了不同类型的应激损伤。本研究旨在评价白术多糖硒（Se）的作用。研究了硒和PAMK对热应激（HS）鸡脾脏氧化应激、内质网应激和细胞凋亡的影响。1日龄雏鸡200只，随机分为对照组（Con）和HS组。然后，这些鸡被单独用Se（0.3 mg kg−1）、PAMK（200 mg kg−1）以及Se（0.3 mg kg−1）和PAMK（200 mg kg−1）的组合处理。采用荧光定量PCR或western blot方法检测诱导型一氧化氮合酶（iNOS）-NO活性（包括NO含量和iNOS活性）、内质网应激相关基因（GRP 78、GRP 94、ATF 4、ATF 6和IRE）和凋亡相关基因（caspase 3和Bcl-2）。还使用超微结构观察来测量细胞凋亡水平。结果表明，HS诱导了鸡脾脏NO含量和iNOS活性的升高，表明HS诱导了鸡脾脏的氧化应激。ER应激相关基因（GRP 78、GRP 94、ATF 4、ATF 6和IRE）和凋亡相关基因caspase 3表达增加（P < 0.05），Bcl-2表达降低（P < 0.05）。硒、PAMK单独或联合应用均能减轻上述反应（P < 0.05或P < 0.05）。结果表明，在正常条件下，Se和PAMK可通过降低氧化应激、内质网应激和细胞凋亡水平来改善免疫应答;而在HS条件下，Se和PAMK可通过改变氧化应激、内质网应激和细胞凋亡的异常水平来减轻HS所致的损伤。硒和PAMK对鸡脾脏中这些生物标志物的调节存在协同作用。硒和PAMK可能在调节鸡免疫功能中起重要作用。
Endoplasmic reticulum (ER) stress and oxidative stress are involved in different types of stress induced injuries. The aim of the present study was to evaluate the effect of selenium (Se), polysaccharide of Atractylodes macrocephala Koidz. (PAMK), and the combination of Se and PAMK on oxidative stress, ER stress and apoptosis in chicken spleen under heat stress (HS) condition. Chickens (1 day old, n = 200) were randomly divided into two groups, the control (Con) group and the HS group. Then these chickens were treated with Se (0.3 mg kg−1), PAMK (200 mg kg−1) alone, and the combination of Se (0.3 mg kg−1) and PAMK (200 mg kg−1). The inducible NO synthase (iNOS)–NO activities (including NO content and iNOS activity), ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and apoptosis-related genes (caspase3 and Bcl-2) were examined by fluorescence quantitative PCR or western blot analysis. Apoptosis levels were also measured using ultrastructural observations. The results indicated that HS induced higher levels of NO content and the activity of iNOS which demonstrated that oxidative stress occurred in the chicken spleen. The ER stress-related genes (GRP78, GRP94, ATF4, ATF6 and IRE) and the apoptosis related gene caspase3 were increased (P < 0.05), while Bcl-2 was decreased (P < 0.05) induced by heat stress. These responses were alleviated by the treatment of Se, PAMK alone, and the combination of Se and PAMK (P < 0.05 or not). The results showed that under common conditions, Se and PAMK could improve the immune response by reducing the levels of oxidative stress, ER stress and apoptosis to proper levels; however, under HS condition, Se and PAMK could change the abnormal levels of oxidative, ER damage and apoptosis to ameliorate the injury induced by HS. In addition, there existed a synergistic effect on the modulation of these biomarkers in chicken spleen between Se and PAMK. So both Se and PAMK may play important roles in regulating the immune function in chicken.
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影响因子：
3.7
作者：
Slawinska A;Hsieh JC;Schmidt CJ;Lamont SJ
通讯作者：
Lamont SJ
影响因子：
2.7
作者：
Khan, R. U.;Naz, S.;Tufarelli, V.
通讯作者：
Tufarelli, V.
DOI：
10.1074/jbc.m109.014092
发表时间：
2010-02-26
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Armstrong JL;Flockhart R;Veal GJ;Lovat PE;Redfern CP
通讯作者：
Redfern CP
影响因子：
3.9
作者：
Xu, Danning;Li, Wanyan;Tian, Yunbo
通讯作者：
Tian, Yunbo
影响因子：
15.1
作者：
Starkie, RL;Hargreaves, M;Febbraio, MA
通讯作者：
Febbraio, MA