Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.
Superficially porous particles with 1000Å pores for large biomolecule high performance liquid chromatography and polymer size exclusion chromatography.
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DOI：
10.1016/j.chroma.2017.01.082
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发表时间：
2017-03-17
期刊：
影响因子：
--
通讯作者：
Schure MR
中科院分区：
文献类型：
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作者：
Wagner BM;Schuster SA;Boyes BE;Shields TJ;Miles WL;Haynes MJ;Moran RE;Kirkland JJ;Schure MR
为了促进质量传递和柱效率，溶质必须能够自由进入颗粒孔以促进与固定相的相互作用。为了确保这一特性，颗粒应用于HPLC分离，其具有足够大的孔以容纳溶质而不限制扩散。本文描述了表面多孔（也称为Fused-Core®，核壳或多孔壳）颗粒的设计和性能，这些颗粒具有非常大的（1000 μ m）孔，专门用于分离非常大的生物分子和聚合物。DNA片段，单克隆抗体，大蛋白质和大聚苯乙烯标准品的分离被用来说明这些颗粒的有效性，高分辨率的应用程序的效用。
To facilitate mass transport and column efficiency, solutes must have free access to particle pores to facilitate interactions with the stationary phase. To ensure this feature, particles should be used for HPLC separations which have pores sufficiently large to accommodate the solute without restricted diffusion. This paper describes the design and properties of superficially porous (also called Fused-Core®, core shell or porous shell) particles with very large (1000 Å) pores specifically developed for separating very large biomolecules and polymers. Separations of DNA fragments, monoclonal antibodies, large proteins and large polystyrene standards are used to illustrate the utility of these particles for efficient, high-resolution applications.