REMOVAL OF A 67-BASE-PAIR SEQUENCE IN THE NONCODING REGION OF PROTOONCOGENE-FOS CONVERTS IT TO A TRANSFORMING GENE
REMOVAL OF A 67-BASE-PAIR SEQUENCE IN THE NONCODING REGION OF PROTOONCOGENE-FOS CONVERTS IT TO A TRANSFORMING GENE
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DOI：
10.1073/pnas.82.15.4987
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
VERMA, IM
中科院分区：
文献类型：
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作者：
MEIJLINK, F;CURRAN, T;VERMA, IM
原癌基因fos（c-fos）转化成纤维细胞需要病毒长末端重复序列（LTR）的连接和3“-非编码序列的中断。鉴定了一段67个核苷酸的富含A+ T的片段，位于编码结构域下游627-693个碱基对和推定的poly（A）添加位点上游123-189个碱基对，去除该位点赋予c-fos基因转化活性。提出了一种新的调控c-fos基因表达的方法，它可能在体内起作用，以防止该基因成为癌基因。
Transformation of fibroblasts by protooncogene fos (c-fos) requires the linkage of viral long terminal repeat (LTR) sequences and interruption of 3''-noncoding sequences. An A+T-rich stretch of 67 nucleotides was identified, located 627-693 base pairs downstream from the coding domain and 123-189 base pairs upstream from the putative poly(A) addition site, removal of which confers transforming activity to the c-fos gene. A novel regulation of the expression of the c-fos gene is proposed, which may be functional in vivo to prevent the gene from becoming an oncogene.