Role of ethylene and jasmonic acid on rhizome induction and growth in rhubarb (Rheum rhabarbarum L.)
Role of ethylene and jasmonic acid on rhizome induction and growth in rhubarb (Rheum rhabarbarum L.)
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DOI：
10.1007/s11240-010-9861-y
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发表时间：
2011-05
期刊：
影响因子：
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通讯作者：
Usha P. Rayirath;R. Lada;C. Caldwell;S. Asiedu;K. J. Sibley
中科院分区：
文献类型：
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作者：
Usha P. Rayirath;R. Lada;C. Caldwell;S. Asiedu;K. J. Sibley
本试验旨在阐明激素对大黄根茎生长的诱导和调控作用。乙烯是根状茎诱导和发育的关键调控因子。研究了茉莉酸（JA）及其与乙烯的相互作用在根状茎诱导和生长中的作用。乙烯和茉莉酸对离体根状茎的形成有显著的促进作用。相反，乙烯生物合成抑制剂氨基乙氧基乙烯基甘氨酸（AVG）（1.5 μM）在体外抑制多芽丛的根茎诱导，并抑制外源施用乙烯利（1 mg l−1）和JA（10 ng l−1）促进微型根茎形成的刺激作用，进一步证实了内源性乙烯在该过程中的作用。此外，根茎生长显着增强乙烯和JA（乙烯利1毫克l-1和JA 10纳克l-1）的存在下相比，JA单独。这些结果表明，内源乙烯是大黄根茎生长的关键调节因子，茉莉酸可能通过诱导内源乙烯的合成促进根茎的形成。
Experiments were conducted to elucidate the hormonal induction and regulation of rhizome growth in rhubarb (Rheum rhabarbarumL.). It was found that ethylene is the key regulator of rhizome induction and development. The role of jasmonic acid (JA) and its interaction with ethylene in rhizome induction and growth were also examined. Both ethylene and JA have a significant effect on promoting rhizome formation in vitro. Conversely, the ethylene biosynthesis inhibitor aminoethoxyvinylglycine (AVG) (1.5 μM) inhibited rhizome induction in multiple-shoot clumps in vitro, and suppressed the stimulatory effects of exogenously applied ethephon (1 mg l−1) and JA (10 ng l−1) in promoting mini-rhizome formation, further confirming the role of endogenous ethylene in the process. In addition, rhizome growth was significantly enhanced in the presence of both ethylene and JA (ethephon 1 mg l−1and JA 10 ng l−1) compared to JA alone. These results suggest that endogenous ethylene is the key regulator of rhizome growth in rhubarb and JA promotes rhizome formation, possibly through inducing endogenous ethylene synthesis.