Motion tracking of the outer tips of microtubules
Motion tracking of the outer tips of microtubules
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DOI：
10.1016/j.media.2008.04.004
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发表时间：
2008-12-01
影响因子：
10.9
通讯作者：
Duncan, James
中科院分区：
文献类型：
--
作者：
Hadjidemetriou, Stathis;Toomre, Derek;Duncan, James
微管是细胞质的管状生物聚合物。它们在细胞中发挥着许多关键作用，例如为染色体、细胞器和囊泡的锚定和运输提供机械支撑和结构轨道。它们还形成微管组件，这对于有丝分裂和细胞迁移的协调至关重要。组件中最具活力的部分是微管外部以及靠近细胞皮层的尖端。该组件的异常功能与神经退行性疾病和癌症等细胞病理学有关。迄今为止，微管组件动力学的研究通常通过目视检查来定性地进行，或者通过手动注释图像序列中尖端随时间的位置来定量地进行，这是非常乏味的。在这项工作中，我们开发了一种自动跟踪微管尖端的方法，以便对微管组件进行更广泛和更高通量的定量研究。我们的方法首先使用整个图像序列来估计尖端振荡的区域。在该区域中，始终计算尖端特征并随后用于形成尖端轨迹。最后，我们使用虚拟数据和真实数据评估我们的方法。真实数据显示用落射荧光显微镜或共聚焦显微镜成像的荧光标记活细胞。 (C) 2008 Elsevier B.V. 保留所有权利。
Microtubules are tubular biopolymers of the cytoplasm. They play numerous critical roles in a cell such as providing mechanical support and structural tracks for the anchoring and transport of chromosomes, organelles, and vesicles. They also form the microtubule assembly, which is critical for the coordination of mitosis and cell migration. The most dynamic part of the assembly are the microtubule outer, plus, tips located close to the cortex of the cell. Abnormal function of the assembly has been implicated in cell pathology such as neurodegenerative diseases and cancer. To date the study of the dynamics of the microtubule assembly is often performed qualitatively by visual inspection or quantitatively by manual annotation of the locations of the tips over time in an image sequence, which is very tedious. In this work we have developed a method to automatically track microtubule tips so as to enable a more extensive and higher throughput quantitative study of the microtubule assembly. Our approach first uses the entire image sequence to estimate the region in which a tip oscillates. In that region a tip feature is computed for all time and subsequently used to form the tip trajectory. Last, we evaluate our method with phantom as well as real data. The real data show fluorescently tagged living cells imaged with epifluorescent microscopy or confocal microscopy. (C) 2008 Elsevier B.V. All rights reserved.