Identification of a T cell chemotactic factor in the cerebrospinal fluid of HIV-1-infected individuals as interferon-γ inducible protein 10
Identification of a T cell chemotactic factor in the cerebrospinal fluid of HIV-1-infected individuals as interferon-γ inducible protein 10
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DOI：
10.1016/s0165-5728(98)00223-9
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发表时间：
1999-01-01
影响因子：
3.3
通讯作者：
Fontana, A
中科院分区：
文献类型：
--
作者：
Kolb, SA;Sporer, B;Fontana, A
中枢神经系统（CNS）受累是人类免疫缺陷病毒（HIV-1）感染的一个显著特征。单核细胞和CD 4（+）T细胞穿过血脑屏障（BBB），并通过产生神经毒素作为病毒的载体和脑病理学的肇事者。在本研究中，分析了来自Pmr-l感染患者的脑脊液（CSF）样品中趋化因子的存在。来自患者的所有36个CSF样品对CXC趋化因子干扰素-γ诱导蛋白（IP-IO）呈阳性，而在14个对照的CSF样品中未检测到。LP-IO浓度在伴有HIV-I相关神经系统疾病的HIV-1感染患者中高于无神经系统缺陷的患者。与IP-IO相反，其他趋化因子包括CC趋化因子MCP-1、MIP-1 α、MIP-1 β和RANTES以及细胞因子IL-15和IL-16在不到30%的患者中未检测到或增加。与对照的CSF样品不同，来自HIV-1感染患者的所有CSF样品诱导了用IL-2活化的T细胞的趋化性。IP-10作为T细胞趋化性细胞因子在HIV-1感染CSF中的意义表现为：（1）IP-10水平与T细胞趋化性程度的相关性，（？）抗IP-10抗体对T细胞趋化性的中和作用以及（3）患者CSF样品对活化T细胞的趋化反应与CSF白色细胞计数的相关性。我们的数据提供的证据表明，IP-IO有助于在HIV-1感染者的CSF隔室中积累活化的T细胞。(C)1999 Elsevier Science B. V.保留所有权利。
Central nervous system (CNS) involvement is a prominent feature of human immunodeficiency virus (HIV-I) infection. Monocytes and CD4(+) T cells traverse the blood brain barrier (BBB), and serve as vehicles for the virus and perpetrators for brain pathology by their production of neurotoxins. In the present study cerebrospinal fluid (CSF) samples from Pmr-l-infected patients were analyzed for the presence of chemotactic factors. All 36 CSF samples from the patients were positive for the CXC chemokine interferan-gamma inducible protein (IP-IO), which was not detected in CSF samples of 14 controls. The LP-IO concentrations were higher in HIV-1-infected patients with HIV-I associated neurologic disorders than in those without neurological deficits. In contrast to IP-IO, other chemotactic factors including the CC chemokines MCP-I, MIP-1 alpha, MIP-1 beta and RANTES and the cytokines IL-15 and IL-16 were either not detected or increased in only less than 30% of the patients. Unlike the CSF samples of controls, all CSF samples from HIV-1-infected patients induced chemotaxis of T cells activated with IL-2. The significance of IP-10 as a T cell chemotactic cytokine in HIV-1-infected CSF is shown by (1) the correlation of the IP-10 levels with the extent of T cell chemotaxis, (?) the neutralization of T cell chemotaxis by anti-IP-10 antibodies and (3) the correlation of the chemotactic response of CSF samples on activated T cells and the CSF white cell count in the patients. Our data provide evidence that IP-IO contributes to the accumulation of activated T cells in the CSF compartment in HIV-1-infected individuals. (C) 1999 Elsevier Science B.V. All rights reserved.