Plasticity in the expression of the steroid receptor coactivator 1 in the Japanese quail brain: Effect of sex, testosterone, stress and time of the day
Plasticity in the expression of the steroid receptor coactivator 1 in the Japanese quail brain: Effect of sex, testosterone, stress and time of the day
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DOI：
10.1016/j.neuroscience.2006.03.002
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发表时间：
2006-01-01
期刊：
影响因子：
3.3
通讯作者：
Balthazart, J.
中科院分区：
文献类型：
--
作者：
Charlier, T. D.;Ball, G. F.;Balthazart, J.
核受体对真核基因组作用的分析突出了辅激活因子对基因转录的重要性。类固醇受体共激活因子-1是一个特别的研究热点，生理学或行为学研究已经证实，它在类固醇和甲状腺受体活性的调节中起着重要作用。然而，关于类固醇受体辅激活因子-1在大脑中表达的调控机制还知之甚少.本研究的目的是通过真实的时间定量聚合酶链反应定量其mRNA和通过蛋白质印迹法定量其相应的蛋白质来确定调节日本鹌鹑类固醇受体辅激活因子-1合成的潜在因素。与我们实验室先前发表的结果相反[Charlier TD，Lakaye B，Ball GF，Balthazart J（2002）The steroid receptor coactivator SRC-1 exhibits high expression in steroid-sensitive brain areas regulating reproductive behaviors in the quail brain. Neuroendocrinology 76：297-315]，我们发现性成熟的女性与男性相比在视前区/下丘脑中具有更高浓度的类固醇受体辅激活因子-1。类固醇受体辅激活因子-1在男性视前区/下丘脑的表达上调睾酮和压力往往会下降。我们还确定了一个显着的相关性，一天中的时间和辅激活因子的表达在视叶，海马，端脑和后脑，但在表达的变化模式作为一天中的时间的函数从一个大脑区域到另一个不同。总之，这些数据支持类固醇受体共激活因子-1不是组成型表达的，而是由类固醇、应激和可能的其他未知因素精细调节的想法。(c)2006年IBRO。由爱思唯尔有限公司出版。保留所有权利。
Analysis of nuclear receptor action on the eukaryotic genome highlights the importance of coactivators on gene transcription. The steroid receptor coactivator-1 in particular is the focus of an intense research and physiological or behavioral studies have confirmed that it plays a major role in the modulation of steroid and thyroid receptors activity. However, little is known about the regulation of steroid receptor coactivator-1 expression the brain. The goal of this study was to determine the potential factors modulating steroid receptor coactivator-1 synthesis in Japanese quail by quantification of its mRNA with real time quantitative polymerase chain reaction and of the corresponding protein via Western blotting. Contrary to previously published results from our laboratory [Charlier TD, Lakaye B, Ball GF, Balthazart J (2002) The steroid receptor coactivator SRC-1 exhibits high expression in steroid-sensitive brain areas regulating reproductive behaviors in the quail brain. Neuroendocrinology 76:297-315], we found here that sexually mature females had a higher concentration of steroid receptor coactivator-1 in the preoptic area/hypothalamus compared with males. Steroid receptor coactivator-1 expression in the male preoptic area/hypothalamus was up-regulated by testosterone and tended to be decreased by stress. We also identified a significant correlation between the time of the day and the expression of the coactivator in the optic lobes, hippocampus, telencephalon and hindbrain but the pattern of changes in expression as a function of the time of the day varied from one brain area to another. Together, these data support the idea that steroid receptor coactivator-1 is not constitutively expressed but rather is finely regulated by steroids, stress and possibly other unidentified factors. (c) 2006 IBRO. Published by Elsevier Ltd. All rights reserved.