Significance of 12S Toxin of Clostridium botulinum Type E
Significance of 12S Toxin of Clostridium botulinum Type E
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E型肉毒梭菌12S毒素的意义
DOI：
10.1128/jb.98.3.1173-1178.1969
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发表时间：
1969
影响因子：
3.2
通讯作者：
G. Sakaguchi
中科院分区：
文献类型：
--
作者：
M. Kitamura;S. Sakaguchi;G. Sakaguchi
从12S毒素（原毒素和胰蛋白酶激活的12S毒素）的理化和生物学特性以及每种12S毒素的Eα和Eβ组分方面讨论了E型肉毒中毒的发病机制。每种12S毒素的分子量为350，000，Eα和Eβ的分子量为150，000。由于12S毒素的结构包含亚基Eα和Eβ，因此在低pH值和高温下比Eα稳定得多。A型19S毒素及其α组分也是如此。Eα组分单独占E型毒素的总毒性。经口或胃肠外给予12S毒素的动物血液中检测到的毒性物质根据分子大小和色谱图鉴定为Eα。由于亚基结构，原毒素在胃中逃避解毒，可能在肠中解离释放Eα组分。吸收后，活化的Eα出现在循环血液中，没有任何进一步的解离或酶消化迹象。
The pathogenesis of type E botulism is discussed as an aspect of the physicochemical and biological properties of 12S toxins (prototoxin and trypsin-activated 12S toxin) and the Eα and Eβ components of each 12S toxin. A molecular weight of 350,000 was determined for each 12S toxin and 150,000 for Eα and Eβ. Owing to the structure comprising the subunits Eα and Eβ, 12S toxins are much more stable than Eα at low pH values and high temperatures. Such was also the case with type A 19S toxin and its α component. The Eα component alone accounts for the total toxicity of type E toxin. The toxic substance detected in the blood of the animals administered 12S toxins orally or parenterally was identified as Eα from the molecular size and the chromatographic pattern. Prototoxin escaping from detoxification in the stomach owing to the subunit structure may undergo dissociation in the intestine to release the Eα component. After absorption, the activated Eα appeared in the circulating blood without any further signs of dissociation or enzymatic digestion.