Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
Deoxyribonucleic Acid Profiling Analysis of 40 Human Thyroid Cancer Cell Lines Reveals Cross-Contamination Resulting in Cell Line Redundancy and Misidentification
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DOI：
10.1210/jc.2008-1102
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发表时间：
2008-11-01
影响因子：
5.8
通讯作者：
Haugen, Bryan R.
中科院分区：
文献类型：
--
作者：
Schweppe, Rebecca E.;Klopper, Joshua P.;Haugen, Bryan R.
背景：来自人类癌症的细胞系为研究疾病机制和开发新的治疗方法提供了关键工具。目的：我们利用短串联重复序列和单核苷酸多态性阵列分析方法对40个已报道的甲状腺癌衍生细胞系进行评估。结果：在测试的40个细胞系中，只有23个细胞系具有独特的遗传学特征。以下几组细胞系可能是同一细胞系的衍生物：BHP5-16、BHP17-10、BHP14-9和NPA87；BHP2-7、BHP10-3、BHP7-13和TPC1；KAT5、KAT10、KAT4、KAT7、KAT50、KAK1、ARO81-1和MRO87-1；以及K1和K2。独特的细胞系包括bCPAP、KTC1、TT2609-C02、FTC133、ML1、WRO82-1、8505C、SW1736、CAL-62、T235、T238、UHTH-104、ACT-1、HTh74、KAT18、TTA1、FRO81-2、HTH7、C643、BHT101和KTC-2。被错误识别的细胞系包括DRO90-1，它与黑色素瘤来源的细胞系A-375匹配。ARO81-1及其衍生物与HT-29结肠癌细胞相匹配，NPA87及其衍生物与M14/MDA-MB-435S黑色素瘤细胞相匹配。结论：在过去的20年里，许多人类细胞系被广泛应用于甲状腺癌领域，它们不仅是多余的，而且不是甲状腺起源的。这些结果强调了细胞系完整性的重要性，并提供了一组甲状腺癌细胞系的短串联重复序列图谱，可作为其他实验室细胞系比较的参考。(J Clin Endocrinol Metab 93：4331-4341,2008)
Context: Cell lines derived from human cancers provide critical tools to study disease mechanisms and develop novel therapies. Recent reports indicate that up to 36% of cell lines are cross-contaminated.Objective: We evaluated 40 reported thyroid cancer-derived cell lines using short tandem repeat and single nucleotide polymorphism array analysis.Results: Only 23 of 40 cell lines tested have unique genetic profiles. The following groups of cell lines are likely derivatives of the same cell line: BHP5-16, BHP17-10, BHP14-9, and NPA87; BHP2-7, BHP10-3, BHP7-13, and TPC1; KAT5, KAT10, KAT4, KAT7, KAT50, KAK1, ARO81-1, and MRO87-1; and K1 and K2. The unique cell lines include BCPAP, KTC1, TT2609-C02, FTC133, ML1, WRO82-1, 8505C, SW1736, Cal-62, T235, T238, Uhth-104, ACT-1, HTh74, KAT18, TTA1, FRO81-2, HTh7, C643, BHT101, and KTC-2. The misidentified cell lines included the DRO90-1, which matched the melanoma-derived cell line, A-375. The ARO81-1 and its derivatives matched the HT-29 colon cancer cell line, and the NPA87 and its derivatives matched the M14/MDA-MB-435S melanoma cell line. TTF-1 and Pax-8 mRNA levels were determined in the unique cell lines.Conclusions: Many of these human cell lines have been widely used in the thyroid cancer field for the past 20 yr and are not only redundant, but not of thyroid origin. These results emphasize the importance of cell line integrity, and provide the short tandem repeat profiles for a panel of thyroid cancer cell lines that can be used as a reference for comparison of cell lines from other laboratories. (J Clin Endocrinol Metab 93: 4331-4341, 2008)