Conjugated diene and trans fatty acids in tissue lipids of rats fed an hepatocarcinogenic choline-devoid diet.
Conjugated diene and trans fatty acids in tissue lipids of rats fed an hepatocarcinogenic choline-devoid diet.
复制标题
喂食致癌胆碱的饮食的大鼠组织脂质中的共轭二烯和反式脂肪酸。
DOI：
10.1093/carcin/11.11.2053
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发表时间：
1990
期刊：
影响因子：
4.7
通讯作者：
Lombardi,B
中科院分区：
文献类型：
--
作者：
Banni,S;Salgo,MG;Evans,RW;Corongiu,FP;Lombardi,B
雄性Fischer-344大鼠组喂食两对半纯化的无胆碱和补充胆碱的饮食，一组含有5%玉米油和10%部分氢化脂肪，另一组仅含有玉米油（15%）。对从大鼠全肝、肝核和脂肪组织中提取的脂质进行分析。分析包括共轭二烯的紫外分光光度法定量，脂肪酸与共轭二烯的HPLC分离和反式脂肪酸的红外分光光度法定量。共轭二烯和共轭二烯和反式脂肪酸在脂肪组织总脂质中观察到，浓度反映了饲料中的浓度。肝脏脂质中的反式脂肪酸也是如此。然而，在喂食仅用玉米油配制的饲料的大鼠的肝脏脂质中未检测到共轭二烯或共轭二烯脂肪酸。与此相反，共轭二烯检测总和中性脂质，但不是在磷脂，整个肝脏和肝细胞核的大鼠喂养的饮食配制部分氢化脂肪。中性脂质含有共轭二烯的脂肪酸，其与膳食部分氢化脂肪中存在的共轭二烯脂肪酸的保留时间一起洗脱。它的结论是，胆碱缺乏饮食，这是在大鼠肝癌，不诱导肝细胞膜脂质过氧化，不仅transfatty酸，但也与共轭二烯存在于部分氢化脂肪的脂肪酸，被吸收和同化在大鼠组织脂质。
Groups of male Fischer-344 rats were fed two pairs of semi-purified choline-devoid and choline-supplemented diets, one set containing 5% corn oil and 10% partially hydrogenated fat, and the other only corn oil (15%). Analyses were performed on lipids extracted from whole liver, liver nuclei and adipose tissue of the rats. The analyses consisted of quantitation of conjugated dienes by UV spectrophotometry, separation of fatty acids with conjugated dienes by HPLC and quantitation oftransfatty acids by IR spectrophotometry. Conjugated dienes and conjugated diene andtransfatty acids were observed in adipose tissue total lipids, at concentrations that reflected those in the diets fed. The same was true oftransfatty acids in liver lipids. However, no conjugated dienes, or fatty acids with conjugated dienes, were detected in liver lipids of rats fed the diets formulated with only corn oil. In contrast, conjugated dienes were detected in total and neutral lipids, but not in phospholipids, of whole liver and liver nuclei of rats fed the diets formulated with partially hydrogenated fat. The neutral lipids contained fatty acids with conjugated dienes that eluted with the retention time of conjugated diene fatty acids, present in the dietary partially hydrogenated fat. It is concluded that a choline-devoid diet, which is hepatocarcinogenic in the rat, does not induce a peroxidation of liver cell membrane lipids, and that not onlytransfatty acids, but also fatty acids with conjugated dienes present in a partially hydrogenated fat, are absorbed and assimilated in rat tissue lipids.