Fruit-specific V-ATPase suppression in antisense-transgenic tomato reduces fruit growth and seed formation
Fruit-specific V-ATPase suppression in antisense-transgenic tomato reduces fruit growth and seed formation
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DOI：
10.1007/s00425-005-0176-x
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发表时间：
2006-05-01
期刊：
影响因子：
4.3
通讯作者：
Shiratake, K
中科院分区：
文献类型：
--
作者：
Amemiya, T;Kanayama, Y;Shiratake, K
液泡是与细胞膨胀、溶质积累、细胞质离子浓度调节、pH稳态和pH调节的过程相关的大的多功能细胞器，其通过液泡H+-泵直接或间接实现：液泡H+-ATP酶（V-ATP酶; EC 3.6.1.3）和液泡H+-焦磷酸酶（V-PPase; EC 3.6.1.1）。本研究以番茄（Lycopersicon esculentum L.）V-ATP酶A亚基，其在果实特异性2A 11启动子的控制下。获得了1个转β-葡萄糖醛酸酶（GUS）基因株系（GUS对照）和7个A亚基反义转基因株系。所有反义转基因株系果实中V-ATPase A亚基mRNA的表达量均下降，但叶片中V-ATPase A亚基mRNA的表达量与GUS对照株系和非转基因株系相比无差异，表明2A 11启动子对V-ATPase A亚基的抑制仅限于果实。反义转基因植株的果实比GUS对照和非转基因植株小。令人惊讶的是，来自反义转基因植物的果实，除了仍然具有相对高的A亚基mRNA表达的果实之外，几乎没有种子。反义转基因植株果实中蔗糖浓度增加，但葡萄糖和果糖浓度没有变化。这些结果表明，V-ATPase不仅在果实生长中，而且在种子形成和番茄果实的糖组成中的重要性。
The vacuole is a large, multifunctional organelle related to the processes of cell expansion, solute accumulation, regulation of cytoplasmic ion concentrations, pH homeostasis and osmoregulation, which are directly or indirectly achieved by vacuolar H+-pumps: vacuolar H+-ATPase (V-ATPase; EC 3.6.1.3) and vacuolar H+-pyrophosphatase (V-PPase; EC 3.6.1.1). In this study, we produced antisense-transgenic tomatoes (Lycopersicon esculentum L.) of the V-ATPase A subunit, which is under the control of the fruit-specific 2A11 promoter. One beta-glucuronidase (GUS)-transgenic line (GUS control) and seven A subunit antisense-transgenic lines were obtained. The amount of V-ATPase A subunit mRNA in fruit decreased in all antisense-transgenic lines, but in leaves showed no difference compared with the GUS control line and the nontransformant, suggesting that suppression of the V-ATPase A subunit by a 2A11 promoter is limited to fruit. The antisense-transgenic plants had smaller fruits compared with the GUS control line and the nontransformant. Surprisingly, fruits from the antisense-transgenic plants, except the fruit that still had relatively high expression of A subunit mRNA, had few seeds. Sucrose concentration in fruits from the antisense-transgenic plants increased, but glucose and fructose concentrations did not change. These results show the importance of V-ATPase, not only in fruit growth, but also in seed formation and in sugar composition of tomato fruit.