Date palm DNA mini-preparation without liquid nitrogen

Date palm DNA mini-preparation without liquid nitrogen
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DOI:
10.1023/a:1007572427029
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发表时间:
1998-09-01
影响因子:
2.1
通讯作者:
Benslimane, A
Benslimane, A
中科院分区:
生物学4区
文献类型:
--
作者:
Ouenzar, B;Hartmann, C;Benslimane, A

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我们开发了一种新的提取枣椰总细胞DNA的小程序。该程序不使用液氮,已被证明是有用的,因为在种植椰枣树的国家发生了液氮供应的暂时中断。获得适合于RFLP和PCR分析的DNA。
We have developed a new mini-procedure for isolation of total cellular DNA from date palm (Phoenix dactylifera L.). The procedure, which does not use liquid nitrogen, has proved useful due to temporary disruptions in supplies of liquid nitrogen that occur in countries where date palm trees are cultivated. DNA suitable for RFLP and PCR analyses is obtained.