Experimental diabetes induced by streptozotocin in the desert gerbil, Gerbillus gerbillus, and the effects of short-term 20-hydroxyecdysone administration.
Experimental diabetes induced by streptozotocin in the desert gerbil, Gerbillus gerbillus, and the effects of short-term 20-hydroxyecdysone administration.
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DOI：
10.1016/j.biopha.2018.03.070
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发表时间：
2018-06
期刊：
影响因子：
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通讯作者：
A. Mallek;J. Movassat;S. Ameddah;Junjun Liu;N. Semiane;Ali Khalkhal;Y. Dahmani
中科院分区：
文献类型：
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作者：
A. Mallek;J. Movassat;S. Ameddah;Junjun Liu;N. Semiane;Ali Khalkhal;Y. Dahmani
本工作的目的是研究链脲佐菌素（STZ; 130 mg/kg）在沙漠沙鼠，沙鼠的影响，并在短期管理的20-羟基蜕皮激素（20 E; 5 mg/kg）的影响进行评估。我们观察到，链脲佐菌素的管理引起血浆葡萄糖的显着增加和胰岛素水平的下降。血脂谱和肝糖原含量也发生了变化。胰腺组织中抗氧化酶活性和丙二醛（MDA）含量增加。此外，组织病理学和免疫组织化学分析显示胰腺退行性损伤，β细胞面积百分比下降。用20 E治疗STZ处理的沙鼠可抵消代谢紊乱并减少脂质过氧化。组织学和免疫组织化学研究显示胰腺结构中度改善。这些发现表明，链脲佐菌素给药诱导沙鼠实验性糖尿病，短期给药20 E对STZ治疗的沙鼠葡萄糖稳态具有有益作用，表明20 E可刺激存活的β细胞释放更多胰岛素。
The present work was aimed at studying the effects of streptozotocin (STZ; 130 mg/kg) in the desert gerbil,Gerbillus gerbillus, and at evaluating the impact of the short-term administration of 20-hydroxyecdysone (20E; 5 mg/kg). We observed that administration of streptozotocin caused a significant increase in plasmatic glucose and a decrease in insulin levels. The plasma lipid profile and liver glycogen content were also altered. The activities of antioxidant enzymes and malondialdehyde (MDA) levels were increased in the pancreatic tissue of STZ-treated gerbils. Moreover, histopathological and immunohistochemical analysis showed degenerative damage in the pancreas with a decline in the percentage area of β-cells. Treatment of STZ-treated gerbils with 20E counteracted metabolic disorders and reduced lipid peroxidation. Histological and immunohistochemical studies showed moderate amelioration in the pancreatic structure. These findings indicate that streptozotocin administration induced experimental diabetes in gerbils and that short-term administration of 20E has beneficial effects in glucose homeostasis in STZ-treated gerbils suggesting that 20E may stimulate surviving β-cells to release more insulin.