Stress response in the ascidian Ciona intestinalis: transcriptional profiling of genes for the heat shock protein 70 chaperone system under heat stress and endoplasmic reticulum stress
Stress response in the ascidian Ciona intestinalis: transcriptional profiling of genes for the heat shock protein 70 chaperone system under heat stress and endoplasmic reticulum stress
复制标题
DOI：
10.1007/s12192-009-0133-x
复制
发表时间：
2010-03-01
影响因子：
3.8
通讯作者：
Wada, Shuichi
中科院分区：
文献类型：
--
作者：
Fujikawa, Tetsuya;Munakata, Takeo;Wada, Shuichi
玻璃海鞘的基因组包含8个HSP 70超家族蛋白基因、36个J蛋白基因、1个J样蛋白基因和3个BAG家族蛋白基因。为了全面了解HSP 70分子伴侣系统中基因的应激反应，采用实时荧光定量RT-PCR技术，对热应激和内质网应激条件下这48个基因的转录谱进行了研究。热应激处理增加了6个HSP 70超家族基因、8个J蛋白家族基因和2个BAG家族基因的mRNA水平。在HSP 70家族的DnaK亚家族的胞质组中，Ci-HSPA 1/6/7-like是唯一的热诱导基因，Ci-HSPA 2/8是唯一的组成型活性基因，与迄今为止已在全基因组范围内检测的其他动物相比，其显示出惊人的简单性。对热应激反应的时间过程和温度依赖性的分析表明，在28 A ° C（从对照温度上移10A ° C）热应激处理1h后，Ci-HSPA 1/6/7样表达的诱导上升到峰值。用Brefeldin A（一种已知作为ER应激诱导剂的药物）进行ER应激处理，增加了4个HSP 70超家族基因和4个J蛋白家族基因的mRNA水平。大多数胁迫诱导基因在玻璃海鞘和脊椎动物之间是保守的，正如它们之间密切的进化关系所预期的那样。本研究首次揭示了玻璃海鞘中HSP 70分子伴侣系统基因的应激反应特征，为全面了解HSP 70分子伴侣系统的功能提供了必要的数据。
The genome of Ciona intestinalis contains eight genes for HSP70 superfamily proteins, 36 genes for J-proteins, a gene for a J-like protein, and three genes for BAG family proteins. To understand the stress responses of genes in the HSP70 chaperone system comprehensively, the transcriptional profiles of these 48 genes under heat stress and endoplasmic reticulum (ER) stress were studied using real-time reverse transcriptase-polymerase chain reaction (RT-PCR) analysis. Heat stress treatment increased the messenger RNA (mRNA) levels of six HSP70 superfamily genes, eight J-protein family genes, and two BAG family genes. In the cytoplasmic group of the DnaK subfamily of the HSP70 family, Ci-HSPA1/6/7-like was the only heat-inducible gene and Ci-HSPA2/8 was the only constitutively active gene which showed striking simplicity in comparison with other animals that have been examined genome-wide so far. Analyses of the time course and temperature dependency of the heat stress responses showed that the induction of Ci-HSPA1/6/7-like expression rises to a peak after heat stress treatment at 28A degrees C (10A degrees C upshift from control temperature) for 1 h. ER stress treatment with Brefeldin A, a drug that is known to act as ER stress inducer, increased the mRNA levels of four HSP70 superfamily genes and four J-protein family genes. Most stress-inducible genes are conserved between Ciona and vertebrates, as expected from a close evolutionary relationship between them. The present study characterized the stress responses of HSP70 chaperone system genes in Ciona for the first time and provides essential data for comprehensive understanding of the functions of the HSP70 chaperone system.