Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors.
Participation of non-zinc finger residues in DNA binding by two nuclear orphan receptors.
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DOI：
10.1126/science.1314418
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发表时间：
1992-04
期刊：
影响因子：
56.9
通讯作者：
Thomas E. Wilson;R. E. Paulsen;Kerstien A. Padgett;Jeffrey Milbrandt
中科院分区：
文献类型：
--
作者：
Thomas E. Wilson;R. E. Paulsen;Kerstien A. Padgett;Jeffrey Milbrandt
类固醇-甲状腺激素受体通常以二聚体的形式与DNA序列结合，这些DNA序列包含被称为半位点的重复元件。NGFI-B是一种早期反应蛋白，也是该受体超家族的孤儿成员，它与只包含一个半位点(5‘-AAAGGTCA-3’)的DNA序列结合。从NGFI-B锌指分离出来的一个结构域被称为A盒，它是识别NGFI-B DNA结合元件5‘端的两个腺嘌呤-胸苷(A-T)碱基对所必需的。此外，孤儿受体H-2区II结合蛋白锌指下游的一个结构域，称为T盒，确定了与雌激素受体一半位点(5‘-AGGTCA-3’)的串联重复序列的结合。
Steroid-thyroid hormone receptors typically bind as dimers to DNA sequences that contain repeated elements termed half-sites. NGFI-B, an early response protein and orphan member of this receptor superfamily, binds to a DNA sequence that contains only one half-site (5'-AAAGGTCA-3'). A domain separate from the NGFI-B zinc fingers, termed the A box, was identified and is required for recognition of the two adenine-thymidine (A-T) base pairs at the 5' end of the NGFI-B DNA binding element. In addition, a domain downstream of the zinc fingers of the orphan receptor H-2 region II binding protein, termed the T box, determined binding to tandem repeats of the estrogen receptor half-site (5'-AGGTCA-3').