Involvement of coronatine-inducible reactive oxygen species in bacterial speck disease of tomato
Involvement of coronatine-inducible reactive oxygen species in bacterial speck disease of tomato
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DOI：
10.4161/psb.4.3.7915
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发表时间：
2009-01-01
影响因子：
2.9
通讯作者：
Bender, Carol L.
中科院分区：
文献类型：
--
作者：
Ishiga, Yasuhiro;Uppalapati, Srinivasa Rao;Bender, Carol L.
丁香假单胞菌 pv.番茄 DC3000 (Pst DC3000) 会产生一种导致缺绿病的植物毒素冠菌素 (COR)，该毒素在植物中具有多种毒力功能。番茄叶上细菌性斑点病的标志之一是形成被褪绿包围的坏死病斑。 COR 诱导的萎黄病在疾病发展中的生理意义尚不清楚。在我们最近在《新植物学家》杂志上发表的文章中，我们证明了 COR 诱导的对光合机制的影响导致活性氧 (ROS) 的积累。与在黑暗或弱光条件下培养的接种幼苗相比，接种 Pst DC3000 并在光照下培养的番茄幼苗表现出更多与疾病相关的坏死细胞死亡。此外，COR 抑制类囊体定位的 Cu/Zn 超氧化物歧化酶 (Cu/Zn SOD) 的表达，但不抑制细胞质定位的 Cu/Zn SOD。在本附录中，我们提出了一个模型，说明 COR 作为不同细胞位点植物 ROS 产生的调节剂，导致番茄细菌斑点期间与疾病相关的坏死细胞死亡。
Pseudomonas syringae pv. tomato DC3000 (Pst DC3000) produces a chlorosis-inducing phytotoxin coronatine (COR), which has multiple virulence functions in planta. One of the hallmarks of bacterial speck disease on tomato leaves is the formation of necrotic lesions surrounded by chlorosis. The physiological significance of COR-induced chlorosis in disease development is still unknown. In our recent publication in New Phytologist, we demonstrated that COR-induced effects on photosynthetic machinery resulted in the accumulation of reactive oxygen species (ROS). Tomato seedlings inoculated with Pst DC3000 and incubated in light showed more disease-associated necrotic cell death than inoculated seedlings incubated in either dark or low light conditions. Furthermore, COR suppressed the expression of thylakoid-localized Cu/Zn superoxide dismutase (Cu/Zn SOD), but not the cytosolic-localized Cu/Zn SOD. In this addendum, we propose a model for the function of COR as a regulator of plant ROS production in different cellular sites leading to disease-associated necrotic cell death during bacterial speck of tomato.