Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds
Stimulation of osteoblast responses to biomimetic nanocomposites of gelatin-hydroxyapatite for tissue engineering scaffolds
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DOI：
10.1016/j.biomaterials.2005.01.047
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Salih, V
中科院分区：
文献类型：
--
作者：
Kim, HW;Kim, HE;Salih, V
以胶原蛋白为原料，制备了明胶/羟基磷灰石（HA）纳米复合材料，用于硬组织工程支架材料。在体外成骨细胞反应的纳米复合材料进行了评估，与那些传统的混合明胶-HA复合材料。介绍了一种三维培养方法，包括在培养基中漂浮细胞，以帮助细胞的初始附着到支架上，并检查细胞的增殖和分化行为。成骨细胞MG 63细胞附着在纳米复合材料上的程度明显更高，随后增殖更多。碱性磷酸酶（ALP）活性和骨钙素的细胞产生的纳米复合支架上显着高于传统的复合支架。认为纳米复合材料上的这些改善的细胞反应是由于纳米复合材料上增加的离子释放和血清蛋白吸附，其源自不同的结构和形态特征，即，纳米复合材料支架比传统支架保留了较少结晶和较小尺寸的磷灰石晶体以及更发达的孔结构。基于这些发现，生物仿生合成的纳米复合支架被认为在硬组织再生和组织工程领域具有潜在的用途。(c)2005爱思唯尔有限公司保留所有权利。
Collagen-derived gelatin/hydroxyapatite (HA) nanocomposites were biomimetically synthesized for hard tissue engineering scaffold. In vitro osteoblastic cellular responses to the nanocomposites were assessed in comparison with those conventionally mixed gelatin-HA composites. A three-dimensional culture method involving floating cells in a Culture medium was introduced to assist in the initial attachment of the cells to the scaffolds, and the proliferation and differentiation behaviors of the cells were examined. The osteoblastic MG63 cells attached to the nanocomposites to a significantly higher degree and subsequently proliferated more. The alkaline phosphatase (ALP) activity and osteocalcin produced by the cells were significantly higher on the nanocomposite scaffolds than on the conventional composite scaffolds. These improved cellular responses on the nanocomposites are considered to result from the increased ionic release and serum protein adsorption on the nanocomposites, which was derived from the different structural and morphological characteristics, i.e., the nanocomposite scaffolds retained less-crystallized and smaller-sized apatite crystals and a more well-developed pore configuration than the conventional ones. Based on these findings, the biomimetically synthesized nanocomposite scaffolds are believed to be potentially useful in hard tissue regeneration and tissue engineering fields. (c) 2005 Elsevier Ltd. All rights reserved.