Impact of methimazole treatment on magnesium concentration and lymphocytes activation in adolescents with Graves' disease.
Impact of methimazole treatment on magnesium concentration and lymphocytes activation in adolescents with Graves' disease.
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DOI：
10.1007/s12011-013-9690-z
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发表时间：
2013-06
影响因子：
3.9
通讯作者：
Rolinski, Jacek
中科院分区：
文献类型：
--
作者：
Klatka, Maria;Grywalska, Ewelina;Partyka, Malgorzata;Charytanowicz, Malgorzata;Rolinski, Jacek
本研究旨在观察青少年Graves病（GD）甲亢患者血浆镁（Mg）浓度、活化T CD 4+、T CD 8+淋巴细胞和B淋巴细胞的变化，并探讨他巴唑（MMI）治疗前后上述指标的变化。用流式细胞仪测定60例GD患者确诊时及甲状腺激素水平恢复正常后T、B细胞活化率，用分光光度法测定血浆镁浓度。对照组为20名健康志愿者。与健康对照组相比，治疗前研究组血浆镁浓度较低，活化T和B淋巴细胞频率较高，MMI诱导的甲状腺功能正常活化T CD 3+、T CD 4+、T CD 8+和B CD 19+淋巴细胞百分比之间呈显著负相关，与治疗前血浆镁浓度呈显著正相关（r <-0.335，p < 0.002）。治疗后，GD患者和对照组之间的血浆Mg浓度和活化细胞百分比无显著差异，但CD 8 + CD 25+细胞除外（p = 0.03）。本研究表明活化的T、B细胞在GD的发病中均起重要作用，且活化程度与血浆镁水平有关。使用MMI治疗GD引起的甲状腺功能亢进导致活化淋巴细胞频率降低和镁水平正常化。
The aim of this research was to assess plasma magnesium (Mg) concentration, the frequencies of activated T CD4+ and T CD8+ lymphocytes and B lymphocytes in adolescents with hyperthyroidism due to Graves’ disease (GD), and to assess changes in the above-mentioned parameters during methimazole (MMI) treatment. The frequencies of activated T and B cells were measured by flow cytometry method and plasma Mg concentration was determined by spectrophotometry method in 60 adolescents at the time of GD diagnosis and after receiving the normalisation of the thyroid hormones levels. The control group consisted of 20 healthy volunteers. We observed lower plasma Mg concentration, and higher frequencies of activated T and B lymphocytes in the study group before the treatment in comparison with healthy controls, and with study group in MMI-induced euthyreosis (p < 0.01).Statistically significant negative correlations between the percentages of activated T CD3+, T CD4+, T CD8+ and B CD19+ lymphocytes, and plasma Mg concentration before the treatment were found (r < −0.335, p < 0.002). After the treatment no vital differences in plasma Mg concentration, and in percentages of activated cells between GD patients and controls were found, except CD8+CD25+ cells (p = 0.03). The present study demonstrates that both activated T and B cells might play an important role in the pathogenesis of GD, and activation is related to Mg plasma level. The use of MMI in treatment of hyperthyroidism due to GD leads to decrease the frequencies of activated lymphocytes and normalisation of Mg levels.
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