Mulberry Leaf Extract Reduces Postprandial Hyperglycemia with Few Side Effects by Inhibiting α-Glucosidase in Normal Rats
Mulberry Leaf Extract Reduces Postprandial Hyperglycemia with Few Side Effects by Inhibiting α-Glucosidase in Normal Rats
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DOI：
10.1089/jmf.2010.1368
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发表时间：
2011-07-01
影响因子：
2.4
通讯作者：
Jang, Hae-Dong
中科院分区：
文献类型：
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作者：
Kim, Gyo-Nam;Kwon, Young-In;Jang, Hae-Dong
桑树（Morus alba L.）叶提取物（MLE）是一种有效的植物源性α -葡萄糖苷酶抑制剂，具有较低的α -淀粉酶抑制活性。在121℃的高压灭菌器中加热15分钟制备MLE，并研究其体外和体内抗高血糖活性。通过测定盲肠重量、体积、大便颜色、盲肠淀粉含量、肠道运输能力完整性等指标，分析MLE的不良反应。与阿卡波糖相比，MLE对肠道α -葡萄糖苷酶的体外抑制活性较强，对肠道α -淀粉酶的体外抑制活性较弱。淀粉、麦芽糖和蔗糖的糖负荷试验表明，MLE可能通过作为肠道α -葡萄糖苷酶抑制剂来降低餐后血糖升高。饲养试验表明，MLE可能比其他α -葡萄糖苷酶抑制剂表现出更少的不良副作用，如腹部胀气和流星，这是由于肠道α -淀粉酶的强烈抑制导致淀粉消化受损。这些结果表明，MLE可用于制药食品的开发，通过抑制肠道α -葡萄糖苷酶，降低副作用，控制糖尿病患者的血糖水平。
Mulberry (Morus alba L.) leaf extract (MLE) was investigated as a potent plant-derived a-glucosidase inhibitor with low alpha-amylase inhibitory activity. MLE was prepared by heating in an autoclave at 121 degrees C for 15 minutes, and its in vitro and in vivo antihyperglycemic activities were investigated. The adverse side effects of MLE were analyzed by measuring the weight and volume of the cecum, stool color, starch content in the cecum, and the integrity of intestinal transporting capacity. The in vitro inhibitory activity of MLE on intestinal alpha-glucosidase was potent and that on intestinal alpha-amylase was very weak compared with acarbose. Sugar loading tests with starch, maltose, and sucrose showed that MLE may reduce postprandial increases in blood glucose by acting as an intestinal alpha-glucosidase inhibitor. Feeding tests suggested that MLE may exhibit fewer adverse side effects than other alpha-glucosidase inhibitors, such as abdominal flatulence and meteorism, which are attributed to the impaired digestion of starch by strong inhibition of intestinal alpha-amylase. These results suggest that MLE could be used in the development of pharmaceutical foods to control the blood glucose levels of diabetic patients by inhibiting intestinal alpha-glucosidase with reduced side effects.