Circular binary segmentation for the analysis of array-based DNA copy number data
Circular binary segmentation for the analysis of array-based DNA copy number data
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DOI：
10.1093/biostatistics/kxh008
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发表时间：
2004-10-01
期刊：
影响因子：
2.1
通讯作者：
Wigler, M
中科院分区：
文献类型：
--
作者：
Olshen, AB;Venkatraman, ES;Wigler, M
DNA序列拷贝数是指基因组某一区域的DNA拷贝数量。癌症进展往往涉及DNA拷贝数的改变。新开发的微阵列技术能够同时测量基因组数千个位点的拷贝数。我们对二元分割法进行了改进，称之为环形二元分割法，用于将有噪声的强度测量值转化为具有相同拷贝数的区域。该方法通过模拟进行了评估，并在具有已知拷贝数改变的细胞系数据以及一个乳腺癌细胞系数据集上得到了验证。
DNA sequence copy number is the number of copies of DNA at a region of a genome. Cancer progression often involves alterations in DNA copy number. Newly developed microarray technologies enable simultaneous measurement of copy number at thousands of sites in a genome. We have developed a modification of binary segmentation, which we call circular binary segmentation, to translate noisy intensity measurements into regions of equal copy number. The method is evaluated by simulation and is demonstrated on cell line data with known copy number alterations and on a breast cancer cell line data set.