Biodegradation of Crystalline and Nonaqueous Phase Liquid-Dissolved ATRAZINE by Arthrobacter sp. ST11 with Cd2+ Resistance
Biodegradation of Crystalline and Nonaqueous Phase Liquid-Dissolved ATRAZINE by Arthrobacter sp. ST11 with Cd2+ Resistance
复制标题
节杆菌对结晶和非水相液体溶解的莠去津的生物降解。
DOI：
10.3390/catal12121653
复制
发表时间：
2022-12
期刊：
影响因子：
3.9
通讯作者：
Tao Pan
中科院分区：
文献类型：
--
作者：
Jiamen Zhang;Zhiliang Yu;Yaling Gao;Meini Wang;Kai Wang;Tao Pan
从我国镉污染土壤中分离到一株新的抗镉细菌，该菌株能以阿特拉津为唯一碳源、氮源和能源，在无机盐培养基中生长。经16 S rRNA基因序列分析和理化试验，将该菌鉴定为节杆菌属（Arthrobacter sp.），命名为ST 11。研究了ST 11对阿特拉津的生物降解作用，以晶体形式存在，以非水相液体形式存在于邻苯二甲酸二（2-乙基己基）酯（DEHP）中。48 h后，ST 11消耗MSM中68%的结晶阿特拉津。在相同条件下，经DEHP溶解后，阿特拉津的降解率降低到55%。显然，NAPL溶解的阿特拉津的生物利用度低于结晶阿特拉津。在Luria-Bertani培养基中，0.05- 1.5mmol/L的Cd ~（2+）对ST_（11）的生长无影响（<0.3mmol/L）、有轻微影响（0.5- 1.0mmol/L）或显著抑制（1.5mmol/L）。相应地，在整个浓度范围内（0.05-1.5 mmol/L），Cd 2+促进ST 11降解阿特拉津，无论是晶体或溶解在DEHP中。拒绝吸附Cd 2+可能是ST 11细胞高镉抗性的主要机制。研究结果可为阿特拉津和镉复合污染农田土壤的微生物治理提供参考。
A newly isolated cadmium (Cd)-resistant bacterial strain from herbicides-polluted soil in China could use atrazine as the sole carbon, nitrogen, and energy source for growth in a mineral salt medium (MSM). Based on 16S rRNA gene sequence analysis and physiochemical tests, the bacterium was identified as Arthrobacter sp. and named ST11. The biodegradation of atrazine by ST11 was investigated in experiments, with the compound present either as crystals or dissolved in di(2-ethylhexyl) phthalate (DEHP) as a non-aqueous phase liquid (NAPL). After 48 h, ST11 consumed 68% of the crystalline atrazine in MSM. After being dissolved in DEHP, the degradation ratio of atrazine was reduced to 55% under the same conditions. Obviously, the NAPL-dissolved atrazine has lower bioavailability than the crystalline atrazine. Cd2+ at concentrations of 0.05–1.5 mmol/L either had no effect (<0.3 mmol/L), slight effects (0.5–1.0 mmol/L), or significantly (1.5 mmol/L) inhibited the growth of ST11 in Luria-Bertani medium. Correspondingly, in the whole concentration range (0.05–1.5 mmol/L), Cd2+ promoted ST11 to degrade atrazine, whether crystalline or dissolved in DEHP. Refusal to adsorb Cd2+ may be the main mechanism of high Cd resistance in ST11 cells. These results may provide valuable insights for the microbial treatment of arable soil co-polluted by atrazine and Cd.