Effect of Sophoridine on Ca2+ Induced Ca2+ Release During Heart Failure
Effect of Sophoridine on Ca2+ Induced Ca2+ Release During Heart Failure
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槐定碱对心力衰竭过程中 Ca2+ 诱导的 Ca2+ 释放的影响
DOI：
10.33549/physiolres.933052
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发表时间：
2016-01-01
影响因子：
2.1
通讯作者：
Yang, Y. -J.
中科院分区：
文献类型：
--
作者：
Hu, S. -T.;Shen, Y. -F.;Yang, Y. -J.
苦参碱是一种从中药苦参中提取的生物碱提取物，具有抗炎、抗过敏、抗癌、抗心律失常等多种药理作用。然而，槐定对心力衰竭的影响尚不清楚。本研究采用SD （Sprague-Dawley）慢性心力衰竭大鼠模型，研究槐定碱对心力衰竭的影响。形态学结果显示，与心力衰竭组相比，中、高剂量组肌丝排列有序紧密，肌纤维间溶解消失，线粒体嵴排列紧密。我们研究了Ca2+诱导的Ca2+瞬态，并评估了ryanodine受体（RyR2）和l型Ca2+通道（dihydropyridine receptor， DHPR）的表达。我们发现，中剂量组（δ F/F-0= 43.33 +/- 1.92）和高剂量组（δ F/F-0=47.21 +/- 1.25）与心力衰竭组(δ F/F-0=16.7 +/- 1.29， P
Sophoridine is a type of alkaloid extract derived from the Chinese herb Sophora flavescens Ait (kushen) and possess a variety of pharmacological effects including anti-inflammation, antianaphylaxis, anti-cancer, anti-arrhythmic and so on. However, the effect of sophoridine on heart failure has not been known yet. In this study, the effect of sophoridine on heart failure was investigated using Sprague-Dawley (SD) rat model of chronic heart failure. Morphological results showed that in medium and high dose group, myofilaments were arranged orderly and closely, intermyofibrillar lysis disappeared and mitochondria contained tightly packed cristae compared with heart failure group. We investigated the Ca2+ induced Ca2+ transients and assessed the expression of ryanodine receptor (RyR2) and L-type Ca2+ channel (dihydropyridine receptor, DHPR). We found that the cytosolic Ca2+ transients were markedly increased in amplitude in medium (Delta F/F-0= 43.33 +/- 1.92) and high dose groups (Delta F/F-0=47.21 +/- 1.25) compared with heart failure group (Delta F/F-0=16.7 +/- 1.29, P