Distinct activities of interferon-gamma, lymphokine and cytokine differentiation-inducing factors acting on the human monoblastic leukemia cell line U937.
Distinct activities of interferon-gamma, lymphokine and cytokine differentiation-inducing factors acting on the human monoblastic leukemia cell line U937.
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干扰素-γ、淋巴因子和细胞因子分化诱导因子作用于人单核细胞白血病细胞系 U937 的独特活性。
作者：
P. Harris;P. Ralph;P. Litcofsky;M. Moore
人单核细胞白血病细胞系U937被淋巴因子（LK）和其他细胞因子抑制生长并诱导表达成熟单核细胞的各种特征。先前的实验表明，干扰素-γ（IFN-γ）是负责一些，但不是所有的分化诱导作用的U937。为了确定活性的多样性和特异性，研究了以下因素：含有IFN-γ（100单位/ml）的植物血凝素诱导的LK;纯化的IFN-γ;人集落刺激因子1（CSF-1）;和来自人膀胱癌细胞系5637和肝癌细胞系SK-HEP的条件培养基（a）（CM）。LK制剂不含集落刺激活性，而来自5637的CM和SK-HEP均含有粒细胞-巨噬细胞CSF（3000至4000单位/ml），但不含IFN活性。IFN-γ是淋巴因子中主要的免疫球蛋白G Fc受体诱导物质，因为抗干扰素-γ抗体抑制了大部分这种活性。Fc受体诱导活性的其他来源是来自SK-HEP和5637细胞系的CM。当测试高达800单位/ml时，人CSF-1对Fc受体诱导无活性。LK诱导趋化肽受体，但这种诱导是由于IFN-γ以外的因素，因为抗IFN-γ抗体不抑制诱导，并且在与LK中发现的剂量相当的剂量（100单位/ml）下纯化的IFN-γ在测定中没有活性。SK-HEP和5637 CM具有强的趋化肽受体诱导活性，但人CSF-1在高达800单位/ml时无活性。用佛波醇肉豆蔻酸刺激后的过氧化物产生可由LK、LK与抗IFN-γ抗体、5637和SK-HEP处理诱导。IFN-γ（100单位/ml）和CSF-1（800单位/ml）无效。在高于1000单位/ml的浓度下，IFN-γ诱导过氧化物产生。几种酶活性的诱导被确定为成熟的额外措施。例如，通过LK、IFN-γ、5637 CM和佛波醇肉豆蔻酸诱导N-乙酰葡糖醛酸糖苷酶。碱性磷酸酶由LK、IFN-γ、地塞米松和佛波醇肉豆蔻酸诱导。还检查了1，25-二羟基胆钙化醇，其可诱导检查的大多数成熟标志物。结果表明，来自多种来源的非IFN细胞因子对单核细胞白血病细胞具有深刻的分化诱导作用，其模式与IFN-γ不同。（400字处截断摘要）
The human monoblast leukemia line U937 is growth inhibited and induced to express various characteristics of mature monocytes by lymphokines (LK) and other cytokines. Previous experiments have shown that interferon-gamma (IFN-gamma) is responsible for some but not all of the differentiation-inducing effects on U937. To determine the variety and specificity of activity, the following factors were studied: phytohemagglutinin-induced LK that contained IFN-gamma (100 units/ml); purified IFN-gamma; human colony-stimulating factor 1 (CSF-1); and conditioned medium(a) (CM) from the human bladder carcinoma cell line 5637 and the hepatoma cell line SK-HEP. LK preparations contained no colony-stimulating activity, whereas CM from 5637 and SK-HEP both contained granulocyte-macrophage CSF (3000 to 4000 units/ml) but no IFN activity. IFN-gamma is the major immunoglobulin G Fc receptor-inducing species within lymphokine, since anti-interferon-gamma antibody inhibited most of this activity. Other sources of Fc receptor-inducing activity were CM from SK-HEP and 5637 cell lines. Human CSF-1 when tested up to 800 units/ml was inactive for Fc receptor induction. LK induced the chemotactic peptide receptor, but this induction was due to factors other than IFN-gamma as anti-IFN-gamma antibody did not inhibit the induction, and purified IFN-gamma at a dose equivalent to that found in LK (100 units/ml) had no activity in the assay. SK-HEP and 5637 CM had strong chemotactic peptide receptor-inducing activity, but human CSF-1 was inactive up to 800 units/ml. Peroxide production after stimulation with phorbol myristic acid could be induced by LK, LK with anti-IFN-gamma antibody, 5637, and SK-HEP treatment. IFN-gamma (100 units/ml) and CSF-1 (800 units/ml) were ineffective. Peroxide production was induced by IFN-gamma at concentrations above 1000 units/ml. The inducibility of several enzymatic activities was determined as additional measures of maturation. N-Acetylglucuronidase was induced, for example, by LK, IFN-gamma, 5637 CM, and phorbol myristic acid. Alkaline phosphatase was induced by LK, IFN-gamma, dexamethasone, and phorbol myristic acid. 1,25-Dihydroxycholecalciferol was also examined and could induce most of the maturational markers examined. The results demonstrate that non-IFN cytokines from several sources have profound differentiation-inducing effects on monoblast leukemia cells in a pattern different from that of IFN-gamma.(ABSTRACT TRUNCATED AT 400 WORDS)
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DOI：
--
发表时间：
1983
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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