Increased expression of an epidermal stem cell marker, cytokeratin 19, in cutaneous squamous cell carcinoma
Increased expression of an epidermal stem cell marker, cytokeratin 19, in cutaneous squamous cell carcinoma
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DOI：
10.1111/j.1365-2133.2008.08731.x
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发表时间：
2008-10-01
影响因子：
10.3
通讯作者：
Furue, M.
中科院分区：
文献类型：
--
作者：
Chen, S.;Takahara, M.;Furue, M.
细胞角蛋白19（CK 19）被认为是毛囊隆突处表皮干细胞的一个假定标记。大量研究表明，表皮干细胞在皮肤癌变过程中起重要作用。目的探讨皮肤鳞状细胞癌（squamous cell carcinoma，SCC）中干细胞和增殖细胞的变化及其与SCC的关系。方法30例皮肤肿瘤性皮损，包括光化性角化病（actinic keratosis，AK）、Bowen病（Bowen disease，BD）和SCC各10例，石蜡包埋标本10例，石蜡包埋切片10例。结果30%的AK、50%的BD和80%的SCC组织中CK 19呈阳性反应。CK 19在SCC中的表达水平显著高于AK和BD（P < 0.05）。BD组CK 19阳性细胞数显著高于AK组（P < 0.05）。Ki-67标记指数在AK与BD、AK与SCC之间差异有显著性（P < 0.001），而在BD与SCC之间差异无显著性（P > 0.05）。此外，一项连续切片比较研究表明，在SCC样本的肿瘤细胞亚群中，有少量细胞共表达CK 19和Ki-67。CK 19+细胞的百分比显着相关性与Ki 67+细胞在所有检查的肿瘤性皮肤lessels.Conclusions这些结果表明，CK 19的表达可能与保留干细胞的特性或状态是不致力于终端鳞状分化。
Background Cytokeratin 19 (CK19) has been considered to be a putative marker for epidermal stem cells in the hair follicle bulge. Cumulative reports have shown that epidermal stem cells play an important role in skin carcinogenesis. However, to date there has been no report on the clinical alteration of the stem cells in squamous cell carcinoma (SCC).Objectives To investigate alteration of the stem cells and proliferating cells and to assess their relationship and potential contribution to SCC.Methods Thirty paraffin-embedded neoplastic skin lesions, consisting of 10 cases each of actinic keratosis (AK), Bowen disease (BD) and SCC, were examined immunohistologically for CK19 and Ki-67.Results Positive reactivity for CK19 was seen in 30% of AK, 50% of BD and 80% of SCC lesions. There was significantly higher expression levels of CK19 in SCC than in AK and BD (P < 0.05). In addition, BD lesions harboured a significantly higher number of CK19-positive cells than did AK lesions (P < 0.05). There were significant differences in Ki-67 labelling indices between AK and BD and between AK and SCC (P < 0.001), but not between BD and SCC (P > 0.05). Furthermore, a serial section comparison study showed that there was a minor population of cells co-expressing CK19 and Ki-67 in a subset of the tumour cells of SCC samples. The percentage of CK19+ cells significantly correlated with that of Ki67+ cells in all examined neoplastic skin lesions.Conclusions These results suggest that CK19 expression may be associated with the retention of stem cell characteristics or a state that is uncommitted to terminal squamous differentiation.