Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver
Forty percent and eighty percent methionine restriction decrease mitochondrial ROS generation and oxidative stress in rat liver
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DOI：
10.1007/s10522-008-9130-1
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发表时间：
2008-06-01
期刊：
影响因子：
4.5
通讯作者：
Barja, Gustavo
中科院分区：
文献类型：
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作者：
Caro, Pilar;Gomez, Jose;Barja, Gustavo
饮食限制(DR)可降低啮齿动物线粒体活性氧簇(ROS)的生成和氧化损伤，并延长其最长寿命。蛋白质限制(PR)或蛋氨酸限制(MERT)，而不是脂肪或碳水化合物限制，也会导致这些变化。然而，以前的蛋氨酸试验仅在80%甲硫氨酸水平下进行，并在日粮中用谷氨酸代替日粮中的蛋氨酸。为了明确标准(40%)DR引起的ROS生成减少和氧化应激是否与MERT有关，Wistar大鼠在不改变其他饮食成分的情况下，分别接受40%和80%的MERT。结果发现，与40%PR和40%DR相似，40%和80%的MTR可降低大鼠肝线粒体ROS的生成和自由基漏出百分率，并可降低复合物I和III的浓度、细胞凋亡诱导因子、线粒体DNA的氧化损伤、蛋白质氧化、糖氧化或脂氧化和脂肪酸不饱和度的5种不同指标。结果表明，40%等热能足以降低大鼠肝脏ROS的生成和氧化应激。这表明蛋氨酸摄入量的减少是DR患者氧化应激减少的原因。
Dietary restriction (DR) lowers mitochondrial reactive oxygen species (ROS) generation and oxidative damage and increases maximum longevity in rodents. Protein restriction (PR) or methionine restriction (MetR), but not lipid or carbohydrate restriction, also cause those kinds of changes. However, previous experiments of MetR were performed only at 80% MetR, and substituting dietary methionine with glutamate in the diet. In order to clarify if MetR can be responsible for the lowered ROS production and oxidative stress induced by standard (40%) DR, Wistar rats were subjected to 40% or 80% MetR without changing other dietary components. It was found that both 40% and 80% MetR decrease mitochondrial ROS generation and percent free radical leak in rat liver mitochondria, similarly to what has been previously observed in 40% PR and 40% DR. The concentration of complexes I and III, apoptosis inducing factor, oxidative damage to mitochondrial DNA, five different markers of protein oxidation, glycoxidation or lipoxidation and fatty acid unsaturation were also lowered. The results show that 40% isocaloric MetR is enough to decrease ROS production and oxidative stress in rat liver. This suggests that the lowered intake of methionine is responsible for the decrease in oxidative stress observed in DR.