Salamander-like tail regeneration in the West African lungfish
Salamander-like tail regeneration in the West African lungfish
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DOI：
10.1098/rspb.2019.2939
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发表时间：
2020-02
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通讯作者：
K. M. Verissimo;Louise N. Perez;Aline C. Dragalzew;G. Senevirathne;Sylvain Darnet;W. R. Mendes;Ciro Ariel dos Santos Neves;Erika Monteiro dos Santos;C. N. de Sousa Moraes;Ahmed Elewa-;N. Shubin;N. B. Froebisch;J. de Freitas Sousa;I. Schneider
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作者：
K. M. Verissimo;Louise N. Perez;Aline C. Dragalzew;G. Senevirathne;Sylvain Darnet;W. R. Mendes;Ciro Ariel dos Santos Neves;Erika Monteiro dos Santos;C. N. de Sousa Moraes;Ahmed Elewa-;N. Shubin;N. B. Froebisch;J. de Freitas Sousa;I. Schneider
蝾螈、青蛙蝌蚪和各种蜥蜴都有再生尾巴的非凡能力。古生物学数据表明，这种能力是plesiomorphic，但当尾巴再生的发育和遗传结构出现知之甚少。在这里，我们展示了四足动物尾部再生的形态和分子标志，在西非肺鱼Protopterus annectens，四足动物的姐妹群的生活代表。与蝾螈一样，肺鱼的尾巴再生是通过形成增殖的芽基来进行的，并恢复原始的结构，包括肌肉、骨骼和脊髓。与蜥蜴不同，肺鱼与蝾螈和青蛙相似，肺鱼可以再生脊髓神经元，重建尾部的背腹图案。类似于蝾螈和青蛙蝌蚪，Shh是肺鱼尾巴再生所必需的。通过RNA-seq分析未受伤和再生的尾巴芽基，我们表明，在肺鱼尾巴再生过程中部署的遗传程序与四足动物保持广泛的重叠，与先前涉及两栖动物和蜥蜴尾巴再生的基因和信号通路的上调。此外，肺鱼尾芽基的基因编码的转录后RNA加工组件和转座子衍生的基因显着上调。我们的研究结果表明，四足动物尾部再生的发育过程和遗传程序至少存在于sarcopterygian分支的基部附近，并建立了肺鱼作为再生生物学的有价值的研究系统。
Salamanders, frog tadpoles, and diverse lizards have the remarkable ability to regenerate tails. Paleontological data suggests that this capacity is plesiomorphic, yet when the developmental and genetic architecture of tail regeneration arose is poorly understood. Here we show morphological and molecular hallmarks of tetrapod tail regeneration in the West African lungfish Protopterus annectens, a living representative of the sister group of tetrapods. As in salamanders, lungfish tail regeneration occurs via formation of a proliferative blastema and restores original structures, including muscle, skeleton and spinal cord. In contrast to lizards and similar to salamanders and frogs, lungfish regenerate spinal cord neurons and reconstitute dorsoventral patterning of the tail. Similar to salamander and frog tadpoles, Shh is required for lungfish tail regeneration. Through RNA-seq analysis of uninjured and regenerating tail blastema, we show that the genetic program deployed during lungfish tail regeneration maintains extensive overlap with that of tetrapods, with the upregulation of genes and signaling pathways previously implicated in amphibian and lizard tail regeneration. Furthermore, the lungfish tail blastema showed marked upregulation of genes encoding post-transcriptional RNA processing components and transposon-derived genes. Our results show that developmental processes and genetic program of tetrapod tail regeneration were present at least near the base of the sarcopterygian clade and establish the lungfish as a valuable research system for regenerative biology.