Mass Spectrometry Imaging and Structural Analysis of Lipids Directly on Tissue Specimens by Using a Spiral Orbit Type Tandem Time-of-Flight Mass Spectrometer, SpiralTOF-TOF.
Mass Spectrometry Imaging and Structural Analysis of Lipids Directly on Tissue Specimens by Using a Spiral Orbit Type Tandem Time-of-Flight Mass Spectrometer, SpiralTOF-TOF.
复制标题
使用螺旋轨道型串联飞行时间质谱仪 SpiralTOF-TOF 对组织样本上的脂质进行质谱成像和结构分析。
DOI：
10.5702/massspectrometry.a0013
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发表时间：
2012
影响因子：
--
通讯作者：
M. Toyoda
中科院分区：
文献类型：
--
作者：
Takaya Satoh;A. Kubo;S. Shimma;M. Toyoda
在本文中，我们报道了利用螺旋轨道式和反射式飞行时间质谱仪的组合，通过基质辅助激光解吸/电离串联飞行时间质谱仪直接在组织标本上进行脂类成像和结构分析的方法。从脑组织标本中观察到的最强的质谱峰经串联质谱仪确认为磷脂酰胆碱(34 ： 1)[M+K](+)。电荷远程碎裂通道，这是使用高能碰撞诱导解离观察到的特征，对这一确认做出了重大贡献。通过使用确认的峰进行质量校正，进一步促进了准确的质量分析。在质谱学成像中，我们系统的高分辨率可以分离出差值小于0.1 u的双峰，否则在使用低分辨率反射式飞行时间质谱仪时会出现问题。在m/z 848.56和848.65处观察到的两种化合物在脑组织标本上被发现位于互补位置。这些结果证明了高性能串联飞行时间质谱仪对质谱学成像和观察到的化合物的分析的重要性，从而能够区分生物分子。
In this paper, we report the use of mass spectrometry imaging and structural analysis of lipids directly on a tissue specimen, carried out by means of matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry, using a combination of spiral orbit-type and reflectron-type time-of-flight mass spectrometers. The most intense peak observed in the mass spectrum from a brain tissue specimen was confirmed as phosphatidylcholine (34 : 1) [M+K](+), using tandem mass spectrometry. The charge remote fragmentation channels, which are characteristically observed using high-energy collision-induced dissociation, contributed significantly to this confirmation. Accurate mass analysis was further facilitated by mass correction using the confirmed peak. In mass spectrometry imaging, the high resolving power of our system could separate doublet peak of less than 0.1 u difference, which would otherwise be problematic when using a low-resolution reflectron type time-of-flight mass spectrometer. Two compounds, observed at m/z 848.56 and 848.65, were found to be located in complementary positions on a brain tissue specimen. These results demonstrate the importance of a high-performance tandem time-of-flight mass spectrometer for mass spectrometry imaging and analysis of observed compounds, to allow distinction between biological molecules.
使用多转串联飞行时间质谱仪“MULTUM-TOF/TOF”进行高能碰撞诱导的磷酸肽解离
DOI：
--
发表时间：
2008
期刊：
Journal of Mass Spectrometry (In press)
影响因子：
--
作者：
M.Hirai;S.Kumano;T.-H.Nagai;K.Sudoh;Shimma Shuichi;Shimma Shuichi
通讯作者：
Shimma Shuichi
影响因子：
7.4
作者：
Caprioli, RM;Farmer, TB;Gile, J
通讯作者：
Gile, J