Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants
Effects of DICER-like proteins 2, 3 and 4 on cucumber mosaic virus and tobacco mosaic virus infections in salicylic acid-treated plants
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DOI：
10.1099/vir.0.014555-0
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发表时间：
2009-12-01
影响因子：
3.8
通讯作者：
Carr, John P.
中科院分区：
文献类型：
--
作者：
Lewsey, Mathew G.;Carr, John P.
水杨酸介导的抗性和RNA沉默都是重要的植物抗病毒防御机制。为了研究这些抗性现象之间的重叠，我们研究了缺乏dicer样（DCL）飞壳核糖核酸酶2、3和4的突变拟南芥植物表现sa诱导防御的能力。结果表明，在dc/2/3/4三突变体植株中，外源SA处理刺激了其对黄瓜花叶病毒和烟草花叶病毒两种正感RNA病毒的抗性。我们得出结论，DCL 2、3和4是参与正义RNA病毒沉默的主要DCL环核糖核酸酶，它们不是sa诱导的对这些病毒的有效抗性所必需的。然而，这些发现并不排除RNA沉默对野生型植物中sa介导的抗性有贡献。
Salicylic acid (SA)-mediated resistance and RNA silencing are both important plant antiviral defence mechanisms. To investigate overlap between these resistance phenomena, we examined the ability of mutant Arabidopsis thaliana plants lacking DICER-like (DCL) encloribonucleases 2, 3 and 4 to exhibit SA-induced defence. We found that in dc/2/3/4 triple mutant plants, treatment with exogenous SA stimulated resistance to two positive-sense RNA viruses: cucumber mosaic virus and tobacco mosaic virus. We conclude that DCLs 2, 3 and 4, which are the predominant DCL encloribonucleases involved in silencing of positive-sense RNA viruses, are not required for effective SA-induced resistance to these viruses. However, the findings do not exclude RNA silencing from making a contribution to SA-mediated resistance in wild-type plants.