UM-Chor1: establishment and characterization of the first validated clival chordoma cell line.
UM-Chor1: establishment and characterization of the first validated clival chordoma cell line.
复制标题
DOI：
10.3171/2016.10.jns16877
复制
发表时间：
2018-03
影响因子：
4.1
通讯作者：
Prince MEP
中科院分区：
文献类型：
--
作者：
Owen JH;Komarck CM;Wang AC;Abuzeid WM;Keep RF;McKean EL;Sullivan S;Fan X;Prince MEP
脊索瘤是一种罕见的恶性肿瘤，被认为起源于脊索的残余。它们可以位于沿着中轴骨骼的任何地方，但最常见于斜坡和骶骨尾区，在胎儿发育期间，脊索会退化。脊索瘤对目前的许多治疗方法都有抵抗力，因此手术是主要的治疗方法。癌细胞系可用于在实验室环境中开发新的癌症治疗方法，然后将其转移到临床，但是只有四种经过验证的脉络膜细胞系可用。我们报告成功建立的第五脉络膜细胞系，UM-Chor 1，这是第一个线来自斜坡。通过脉络膜标志物CD 24和brachyury的表型和阳性证实了验证。我们还试图在UM-Chor 1、UCH 1和UCH 2中鉴定ALDH High细胞群，但没有检测到不同的细胞群。UM-Chor 1细胞能够在无血清培养中形成球状体，并且成功地用荧光素酶转导，并且能够在NOD/SCID小鼠中旁骶注射和生长。这种新的斜坡脉络膜细胞系在体外和体内研究的可用性提供了一个机会，在治疗疾病的发展。
Chordomas are rare malignant tumors thought to arise from remnants of the notochord. They can be located anywhere along the axial skeleton, but are most commonly found in the clivus and sacrococcygeal regions, where the notochord regresses during fetal development. Chordomas are resistant to many current therapies, leaving surgery as the primary method of treatment. Cancer cell lines have been useful to develop new cancer treatments in a laboratory setting that can then be transferred to the clinic, but, there are only four validated chordoma cell lines available. We report the successful establishment of the fifth chordoma cell line, UM-Chor1, which is the first line originating from the clivus. Validation was confirmed by phenotype and positivity for the chordoma markers CD24 and brachyury. We also attempted to identify an ALDHHigh cell population in UM-Chor1, UCH1 and UCH2 but did not detect a distinct population. UM-Chor1 cells were able to form spheroids in serum-free culture, and were successfully transduced with luciferase and able to be injected parasacrally and grown in NOD/SCID mice. The availability of this novel clival chordoma cell line for in vitro and in vivo research provides an opportunity for developments in treatment against the disease.