ALTERED UPTAKE OF METRONIDAZOLE INVITRO BY STOCKS OF GIARDIA-INTESTINALIS WITH DIFFERENT DRUG SENSITIVITIES
ALTERED UPTAKE OF METRONIDAZOLE INVITRO BY STOCKS OF GIARDIA-INTESTINALIS WITH DIFFERENT DRUG SENSITIVITIES
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DOI：
10.1016/0035-9203(88)90278-7
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发表时间：
1988-01-01
影响因子：
2.2
通讯作者：
SHEPHERD, RW
中科院分区：
文献类型：
--
作者：
BOREHAM, PFL;PHILLIPS, RE;SHEPHERD, RW
贾第鞭毛虫在含有亚致死浓度甲硝唑的TYI-S-33培养基中生长66周后，选择了一种对药物敏感性降低8倍以上的微生物。这种耐药性是不稳定的，在没有药物的情况下，微生物在生长22周内恢复到其原始敏感性。原始和选择的Unes对[14 C]甲硝唑摄取的比较显示，耐药株系的药物摄取显著降低，表明跨细胞膜的转运机制存在缺陷，或者细胞内甲硝唑还原为活性代谢物的能力降低。9株G.无菌培养。
Growth ofGiardia intestinalisin TYI-S-33 medium containing a sub-lethal concentration of metronidazole over a period of 66 weeks selected a line of organisms that was over 8 times less sensitive to the drug. This resistance was unstable and the organisms reverted to their original sensitivity within 22 weeks of growth in the absence of drug. A comparison of the uptake of [14C]metronidazole by the original and selected Unes showed a highly significant reduction in drug uptake by the resistant line, indicating either a defective transport mechanism across the cell membrane or decreased reduction of metronidazole to the active metabolite within the ceell. There was no significant difference in the uptake of metronidazole by 9 recent isolates ofG. intestinalismaintained in axenic culture.