Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts.
Comparison of the accuracy of two conventional phenotypic methods and two MALDI-TOF MS systems with that of DNA sequencing analysis for correctly identifying clinically encountered yeasts.
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比较两种常规表型方法和两个MALDI-TOF MS系统与DNA测序分析的准确性，以正确识别临床上遇到的酵母菌。
DOI：
10.1371/journal.pone.0109376
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Hsueh PR
中科院分区：
文献类型：
--
作者：
Chao QT;Lee TF;Teng SH;Peng LY;Chen PH;Teng LJ;Hsueh PR
我们评估了两种市售基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）系统（Bruker Biotyper和Vitek MS）和两种传统表型方法（Phoenix 100 YBC和Vitek 2 Yeast ID）的物种水平鉴定的准确性，以及200种常见酵母临床分离株中rDNA基因测序分析的准确性。200株酵母菌对近平滑念珠菌复合群、近平滑念珠菌复合群、近平滑念珠菌guilliermondii complex和C.通过Bruker Biotyper、Vitek MS（使用体外装置[IVD]数据库）、Phoenix 100 YBC和Vitek 2 Yeast ID（沙氏葡萄糖琼脂）系统测定的葡萄糖（葡萄糖）水平分别为92.5%、79.5%、89%和74%。另外72株C.近平滑复合体18株（近平滑复合体各30株）;近平滑病、C. metapsilosis和C.也分别进行了评价。Bruker Biotyper系统能准确鉴定所有的C.近平滑复合体到物种水平。采用Vitek 2 MS（IVD）系统，对所有C.而C. metapsilosis或C.可以准确地识别正骨病。89株酵母菌经Vitek 2 MS（IVD）系统鉴定后，有39株（43.8%）被错误鉴定，其中27株为C.使用Vitek MS Plus SARAMIS数据库，仅用于研究用途，可以正确鉴定直丝酵母菌分离株。结果表明，Vitek 2 MS对所有酵母菌的正确鉴定率提高到87.5%。guilliermondii complex（C. guilliermondii和C.通过Bruker Biotyper系统产生的光谱的聚类分析正确地鉴定了发酵菌株。基于目前的研究结果，MALDI-TOF MS系统为酵母菌的常规鉴定提供了一种有前途的替代方法，包括临床上常见和罕见的酵母菌以及属于C. parapsilosis complex、C. guilliermondii complex和C.皱纹复合体
We assessed the accuracy of species-level identification of two commercially available matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems (Bruker Biotyper and Vitek MS) and two conventional phenotypic methods (Phoenix 100 YBC and Vitek 2 Yeast ID) with that of rDNA gene sequencing analysis among 200 clinical isolates of commonly encountered yeasts. The correct identification rates of the 200 yeast isolates to species or complex (Candida parapsilosis complex, C. guilliermondii complex and C. rugosa complex) levels by the Bruker Biotyper, Vitek MS (using in vitro devices [IVD] database), Phoenix 100 YBC and Vitek 2 Yeast ID (Sabouraud's dextrose agar) systems were 92.5%, 79.5%, 89%, and 74%, respectively. An additional 72 isolates of C. parapsilosis complex and 18 from the above 200 isolates (30 in each of C. parapsilosis, C. metapsilosis, and C. orthopsilosis) were also evaluated separately. Bruker Biotyper system could accurately identify all C. parapsilosis complex to species level. Using Vitek 2 MS (IVD) system, all C. parapsilosis but none of C. metapsilosis, or C. orthopsilosis could be accurately identified. Among the 89 yeasts misidentified by the Vitek 2 MS (IVD) system, 39 (43.8%), including 27 C. orthopsilosis isolates, could be correctly identified Using the Vitek MS Plus SARAMIS database for research use only. This resulted in an increase in the rate of correct identification of all yeast isolates (87.5%) by Vitek 2 MS. The two species in C. guilliermondii complex (C. guilliermondii and C. fermentati) isolates were correctly identified by cluster analysis of spectra generated by the Bruker Biotyper system. Based on the results obtained in the current study, MALDI-TOF MS systems present a promising alternative for the routine identification of yeast species, including clinically commonly and rarely encountered yeast species and several species belonging to C. parapsilosis complex, C. guilliermondii complex, and C. rugosa complex.
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影响因子：
14.2
作者：
Bader, O.;Weig, M.;Kuhns, M.
通讯作者：
Kuhns, M.
影响因子：
9.4
作者：
Borman, Andrew M.;Petch, Rebecca;Johnson, Elizabeth M.
通讯作者：
Johnson, Elizabeth M.
影响因子：
0.8
作者：
Ahmad, Suhail;Khan, Zia U.;Chandy, Rachel
通讯作者：
Chandy, Rachel
影响因子：
2.9
作者：
Sendid, Boualem;Ducoroy, Patrick;Dalle, Frederic
通讯作者：
Dalle, Frederic
DOI：
10.1016/j.ijantimicag.2012.07.022
发表时间：
2012-12-01
影响因子：
10.8
作者：
Chen, Chien-Yuan;Huang, Shang-Yi;Hsueh, Po-Ren
通讯作者：
Hsueh, Po-Ren