A central role for P48/45 in malaria parasite male gamete fertility
A central role for P48/45 in malaria parasite male gamete fertility
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DOI：
10.1016/s0092-8674(01)00199-4
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发表时间：
2001-01-12
期刊：
影响因子：
64.5
通讯作者：
Eling, W
中科院分区：
文献类型：
--
作者：
van Dijk, MR;Janse, CJ;Eling, W
受精和受精卵发育是疟原虫生命周期的固有特征，发生在疟原虫传播给蚊子的过程中。表面蛋白PFS 48/45由恶性疟原虫的雄配子和雌配子表达，并且PFS 48/45抗体阻止合子发育和传播。在这里，基因破坏被用来显示Pfs 48/45和来自啮齿类疟原虫伯氏疟原虫的直系同源物Pbs 48/45在受精中发挥保守和重要的作用。p48/45（-）寄生虫在蚊子中产生卵囊的能力降低，这是由于受精卵形成大大减少。出乎意料的是，只有p48/45（-）寄生虫的雄性配子生育力受到影响，未能穿透其他可育的雌性配子。P48/45被证明是疟原虫的表面蛋白，在受精中具有明显的作用。
Fertilization and zygote development are obligate features of the malaria parasite life cycle and occur during parasite transmission to mosquitoes. The surface protein PFS48/45 is expressed by male and female gametes of Plasmodium falciparum and PFS48/45 antibodies prevent zygote development and transmission. Here, gene disruption was used to show that Pfs48/45 and the ortholog Pbs48/45 from a rodent malaria parasite P. berghei play a conserved and important role in fertilization. p48/45(-) parasites had a reduced capacity to produce oocysts in mosquitoes due to greatly reduced zygote formation. Unexpectedly, only male gamete fertility of p48/45(-) parasites was affected, failing to penetrate otherwise fertile female gametes. P48/45 is shown to be a surface protein of malaria parasites with a demonstrable role in fertilization.