Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
Degradation of endogenous proteins and generation of a null-like phenotype in zebrafish using Trim-Away technology
复制标题
使用 Trim-Away 技术降解斑马鱼内源蛋白并产生无效表型
DOI：
10.1186/s13059-019-1624-4
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发表时间：
2019-01-23
期刊：
影响因子：
12.3
通讯作者：
Tang, Kai-Fu
中科院分区：
文献类型：
--
作者：
Chen, Xiao;Liu, Mi;Tang, Kai-Fu
Trim-Away是一项最近的技术，可以快速从任何类型的细胞中消耗蛋白质。在抗体的引导下，TRIM21选择蛋白质进行破坏。然而，这种方法在模式生物中的适用性尚未得到研究。在这里，我们证明Trim-Away可以降解斑马鱼胚胎中的蛋白质。Trim-Away比morpholinos更快地消耗蛋白质，这使得分析早期胚胎发生过程中的蛋白质功能成为可能。此外，Trim-Away可用于评估母体贡献蛋白在斑马鱼胚胎中的作用。我们的研究结果表明，Trim-Away是斑马鱼发育过程中蛋白质功能分析的有力工具。
Trim-Away is a recent technique to rapidly deplete a protein from any cell type. Guided by antibodies, TRIM21 selects proteins for destruction. However, the applicability of this method in model organisms has not been investigated. Here, we show that Trim-Away can degrade proteins in zebrafish embryos. Trim-Away depletes proteins faster than morpholinos, which enables analysis of protein function during early embryogenesis. Furthermore, Trim-Away can be applied to evaluate the role of maternally contributed proteins in zebrafish embryos. Our findings indicate that Trim-Away is a powerful tool to perform functional analysis of proteins during zebrafish development.