Encapsulation of islets with ultra-thin polyion complex membrane through poly(ethylene glycol)-phospholipids anchored to cell membrane
Encapsulation of islets with ultra-thin polyion complex membrane through poly(ethylene glycol)-phospholipids anchored to cell membrane
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DOI：
10.1016/j.biomaterials.2006.07.039
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发表时间：
2006-12-01
期刊：
影响因子：
14
通讯作者：
Iwata, Hiroo
中科院分区：
文献类型：
--
作者：
Miura, Suguru;Teramura, Yuji;Iwata, Hiroo
朗格汉斯胰岛（胰岛）的微囊化已被研究为一种安全且简单的胰岛移植技术，无需免疫抑制治疗。然而，需要更薄的膜，因为植入物总体积的增加导致移植部位有限。在这里，我们提出了一种通过在胰岛上形成聚离子复合膜进行微囊化的新方法。当将氨基封端的聚乙二醇结合磷脂（PEG-脂质，M-W：5000）添加到胰岛悬浮液中时，它们会在胰岛外层的细胞上自发形成薄层。通过PEG链末端的氨基、海藻酸钠和聚(L-赖氨酸)之间形成聚离子复合物，可以在PEG-脂质层上进一步形成这种层层膜。通过这种方法可以将胰岛微囊化而不增加体积。用 PEG-脂质和聚离子复合膜封装胰岛表面不会损害响应葡萄糖刺激的胰岛素释放功能。我们的方法有望封装胰岛而不影响细胞活力或增加体积。 (c) 2006 Elsevier Ltd. 保留所有权利。
The microencapsulation of islets of Langerhans (islets) has been studied as a safe and simple technique for islet transplantation without the need for immuno-suppressive therapy. However, thinner membranes are desired, because the increased total volume of the implant led to limited transplantation sites. Here, we propose a novel method for microencapsulation by polyion complex membrane formation on islets. Amino group-terminated poly(ethylene glycol)-conjugated phospholipids (PEG-lipids, M-W: 5000) spontaneously formed a thin layer on cells existing in the outer layer of islets when they were added to islet suspension. This layer-by-layer membrane could be further formed on the PEG-lipid layer through polyion complex formation between amino groups at the end of PEG chains, sodium alginate and Poly(L-lysine). Islets could be microencapsulated by this method without volume increase. Encapsulation of the islet surface with PEG-lipids and polyion complex membranes did not impair the insulin release function in response to glucose stimulation. Our method is promising to encapsulate islets without affecting cell viability or increasing volume. (c) 2006 Elsevier Ltd. All rights reserved.